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Evaluation of In Vivo Transfection Efficiency of Eudragit Coated Nanoparticles of Chitosan-DNA: A pH-sensitive System Prepared for Oral DNA Delivery
Gene Therapy Delivery of DNA Nanoparticles
2015/10/22
Background: Success of any gene therapy protocol relies mostly on using an efficient carrier to direct nucleic acid to the place of action. The system should also have transfection ability at release ...
Transfection of BoHV-5 DNA with calcium phosphate in different types of cell culture
BoHV-5 calcium phosphate transfection calf testicle cells
2010/9/21
The conditions for an efficacious transfection by calcium phosphate method can vary substantially. In this study the most appropriate cell type for transfection of bovine herpesvirus type 5 (BoHV-5) D...
Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
blastoderm electrode electroporation GFP gene
2009/7/31
In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (...
Transfection of Human Kidney Epithelial 293 Cells With Bacterial Cytosine-5-Methyltransferase M.Msp1 and The SV40 Nuclear Localisation Sequence of VP1
Kidney epithelial 293 cells C-5 MTase M.Msp1 NLS-VP1 cell transfection
2009/6/30
The aim of this study was to con-struct a plasmid that would direct the expres-sion of a bacterial DNA methyltransferase in cultured mammalian cells. It was carried out with a PCR-based strategy for i...
Limited Processing of Pro-Matrix Metalloprotease-2（Gelatinase A）Overexpressed by Transfection in PC-3 Human Prostate Tumor Cells: Association With Restricted Cell Surface Localization of Membrane-Type Matrix Metalloproteinase-1
Tissue inhibitor of metalloproteinase-2 prostate cancer Concanavalin A reverse transcriptase-polymerase chain reaction
2008/12/22
The expression and activation of matrix metalloproteinases (MMPs) by tumor cells is correlated with progression to invasive and metastatic status. The purpose of this study was to examine the role of ...