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Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens
blood cells tissue CD9
2015/6/8
Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different speci...
Detection of spring viraemia of carp virus (SVCV) with monoclonal antibodies
monoclonal antibodies spring viraemia of carp SVCV diagnostics ELISA
2015/3/13
Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV), a severe disease in cyprinid fish, were prepared. Nine MAbs were characterised using Western blotting (WB) where all reacted ...
A large number of monoclonal antibodies (mAbs) are currently
under investigation for the treatment of malignant diseases. To
date, a significant amount of experimental and clinical research
had bee...
Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.)
Cyprinus carpio IgM monoclonal antibodies ELISA Western blot
2015/3/5
A method for purification of carp serum immunoglobulin (IgM), intended for the production of monoclonal antibodies, was described in the present study. Hybridomas that produce antibodies against IgM h...
Monoclonal antibodies to bovine coronavirus and their use in enzymoimmunoanalysis and immunochromatography
Immunochromatographic test (ICT) ELISA
2015/1/20
Two monoclonal antibodies (MAb) to the outer structural protein E2 (spike peplomeric protein) and two MAb to the inner capsid protein N of bovine coronavirus (BCV) were prepared and identified by West...
Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Production of monoclonal antibodies against Streptococcus mutans antigens
Antibodies monoclonal Streptococcus mutans
2009/10/19
Several studies have been conducted in the last decades aiming to obtain an anti-caries vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens a...
Pharmacologically Active Saikosaponin in Bupleurum falcatum Detected by Competitive ELISA and Eastern Blotting Using Monoclonal Antibodies
Bupleurum falcatum Eastern blotting ELISA Monoclonal antibody Saikosaponin Umbelliferae
2009/7/23
This study showed the effective microanalytic methods for detection of saikosaponin by a competitive enzyme-linked immunosorbent assay (ELISA) using anti-saikosaponin a (SSa) monoclonal antibody (MAb)...
Production and Purification of Monoclonal Antibodies to Human Interleukin-2 and Their Use for Immunoaffinity Purification of Recombinant Human Interleukin-2
Monoclonal antibody human interleukin-2 immunoaffinity purification
2009/6/26
Monoclonal antibodies (mAbs) are a powerful immunochemical tool. Today, the availability of mAbs with desired specificity enables ligand separation based upon the immunoaffinity technique. By using th...
Production, Characterization and Use of Monoclonal Antibodies Recognizing IgY Epitopes Shared by Chicken, Turkey, Pheasant, Peafowl and Sparrow
monoclonal antibodies IgY avian Phasianidae
2009/3/17
Chicken antibodies are not only a part of immune defense but are more and more popular commercial products in form of chicken polyclonal, monoclonal or recombinant antibodies. We produced and characte...
A panel of monoclonal antibodies against human sperm
Antisperm antibodies sperm antigens serum immunolocalization
2009/2/17
To help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm antigens. The immunoglobulin G (IgG) fra...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies：I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...
Use of neonatal tolerization and chemical immunosuppression for the production of monoclonal antibodies to maturation-specific sperm surface molecules
neonatal tolerization chemical immunosuppression monoclonal antibodies
2009/2/7
Mammalian sperm acquire functional maturity as they move from the caput to the cauda epididymidis. Changes occur in the protein/glycoprotein composition of the sperm plasma membrane during this time...
Preparation and characterization of monoclonal antibodies against sperm-specific lactate dehydrogenase C4
LDH-C4 monoclonal antibodies specificity
2009/2/6
LDH-C4, the testis-specific isozymes of lactate dehydrogenase (LDH), is the predominant LDH isozyme in mammalian spermatozoa. Nine monoclonal antibodies against mouse LDH-C4 have been developed. The...