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Luteinizing hormone secretion by male rat pituitary cells perifused in vitro：effect of experimental left varicocele and orchiectomy
Luteinizing hormone secretion male rat pituitary cells
2009/2/23
It has been shown previously that experimental left varicocele in the rat, results in a bilateral decrease in intratesticular testosterone. In the present work, pituitary responsiveness to GnRH as a...
The effect of tumor necrosis factor on human sperm motility in vitro
tumor necrosis factor human sperm motility
2009/2/23
Tumor necrosis factor (TNF alpha) is present in elevated levels in peritoneal fluid from infertile women with endometriosis. The effect of TNF alpha on human sperm motility in vitro was evaluated ...
Evaluation of computerized analysis of sperm movement characteristics and differential sperm tail swelling patterns in predicting human sperm in vitro fertilizing capacity
sperm movement characteristics differential sperm tail predicting human sperm
2009/2/23
Multivariate discriminant analysis was used to evaluate the usefulness of computer image analysis of sperm movement characteristics and differential patterns of sperm tail swelling after hypoosmot...
Characterization and localization of beta-adrenergic receptors in control and cryptorchidized rat testis by in vitro autoradiography
$-adrenergic receptors autoradiography cryptorchidectomy rat testis Sertoli cells
2009/2/23
beta-adrenergic receptors were localized and characterized in control and cryptorchidized rats to investigate further their role in testicular function. Slide-mounted cryostat sections were incubate...
Effect of germ cells on vectorial secretion of androgen binding protein and transferrin by immature rat Sertoli cells in vitro
germ cells vectorial secretion androgen binding protein transferrin immature rat Sertoli cells
2009/2/23
The influence of germ cells (greater than 85% pachytene spermatocytes) on vectorial secretion of androgen binding protein (ABP) and transferrin by immature rat Sertoli cells was investigated using t...
Uptake and release of ascorbic acid by rat Leydig cells in vitro
ascorbic acid rat Leydig cells
2009/2/23
The mechanism by which ascorbic acid is transported into rat Leydig cells in vitro was investigated. Transport of [14C]ascorbic acid was temperature-dependent and inhibited in the absence of sodium ...
Correlation between in vitro fertilization and human sperm density and motility
Correlation fertilization human sperm density motility
2009/2/23
The conventional sperm characteristics of density (millions per milliliter) and motility, scored in a semi-subjective way, were correlated with results of an on-going in vitro fertilization and em...
Correlation between human sperm swelling in hypoosmotic medium（hypoosmotic swelling test）and in vitro fertilization
human sperm hypoosmotic medium（hypoosmotic swelling test） vitro fertilization
2009/2/23
Human ejaculates (n = 83) were analyzed for standard sperm parameters (concentration, motility, and morphology), as well as for the ability of the spermatozoa to react (swell) in a hypoosmotic mediu...
In vitro production of a TRH-homologous peptide and His-Pro diketopiperazine by human semen
TRH-like peptide His-Pro diketopiperazine peptide biosynthesis in semen
2009/2/23
Fresh human semen, diluted 1:1 v/v with 0.15 M NaCl- 0.05 M phosphate, pH 7.5, undergoes a 3-fold increase in total thyrotropin-releasing hormone (TRH) immunoreactivity on incubation at 4 C for 8 to...
In vitro differentiation of rat seminiferous tubular segments from defined stages of the epithelial cycle morphologic and immunolocalization analysis
rat seminiferous tubular segments defined stages epithelial cycle morphologic immunolocalization analysis
2009/2/23
Rat seminiferous tubule segments have been cultured in chemically defined medium (F12/DMEM 1:1) without added hormones or growth factors. The segments (1-2 mm) were isolated from defined stages of t...
Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation
Flow cytometric DNA analysis rat seminiferous epithelial cycle
2009/2/23
The stages of the rat seminiferous epithelial cycle have been isolated for flow cytometric analysis of DNA and for culture, using transillumination-assisted microdissection. Precise stages have been...
Effects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa
white blood cells zona-free hamster eggs human spermatozoa
2009/2/20
The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of no...
Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro
gossypol sperm motility sperm maturation cytoplasmic droplet capacitation acrosome reaction
2009/2/20
To determine the effects of gossypol acetate on guinea pig epididymal and vas deferens sperm maturity and in vivo susceptibility to in vitro capacitation and the acrosome reaction, we examined sperm...
Specific inhibition of the testicular mitochondrial respiratory chain in vitro by gossypol
Specific inhibition testicular mitochondrial respiratory chain gossypol
2009/2/20
Optically inactive gossypol is an effective male antifertility agent in several mammalian species, while optically active (+)-gossypol has no antifertility effect in the rat and hamster. Recently, i...
Protein secretion by the rat epididymis can be selectively modified in vitro by local anesthetics, glucose deprivation, dinitrophenol, ouabain, and ionophores
Protein secretion rat epididymis selectively modified local anesthetics
2009/2/20
Protein secretion by the caput epididymidis has been examined in vitro using radioactive methionine as a precursor for protein synthesis. Newly synthesized and secreted proteins were separated by gel ...