搜索结果: 1-15 共查到“口腔医学 PCR”相关记录16条 . 查询时间(0.078 秒)
近日，广西医科大学附属口腔医院引进的高层次人才（第三层次）蒋兴禄博士在国际顶级期刊Chemical Engineering Journal（中科院一区，IF=16.744）发表题目为“An amplification velocity-controlled PCR device for accurately detect the initial content of target DNA temp...
实时荧光定量PCR法检测原发性Sjögren综合征口腔真菌菌群
干燥综合征 口腔黏膜 真菌 实时聚合酶链反应
2018/6/15
目的：定量分析原发性舍格伦综合征（primary Sjögren’s syndrome, pSS）患者组和健康对照组口腔内真菌菌量，为探寻敏感、快速和准确的pSS诊断方法及其临床早期诊断和防治提供有价值的参考依据。方法：研究对象为北京大学口腔医院黏膜科25名pSS患者（pSS组）和北京某社区25名无口干症状身体健康的居民（健康对照组），收集两组受试者的唾液含漱液样本，采用实时荧光PCR方...
多重PCR技术在正畸治疗患者口腔细菌检测中的应用
固定矫治器 多重PCR 细菌检测
2012/7/4
建立多重PCR方法检测正畸治疗患者口腔中伴放线放线杆菌（Aa）、福塞氏类杆菌(Bf)、具核梭杆菌(Fn)、牙龈卟啉单胞菌(Pg)4种细菌的存在，并与牙龈指数进行相关性分析。方法 选择55例正畸治疗至少2个月的青少年患者为矫治组，34例未带矫治器的牙周健康者为对照组，记录牙龈指数，分别采龈沟液标本进行细菌DNA提取及聚合酶链式反应；同时，以细菌的厌氧培养和生化反应鉴定为标准进行对比验证，并做了PC...
Epstein-Barr virus in oral hairy leukoplakia scrapes:identification by PCR
Epstein-Barr virus infections Leukoplakia,hairy Polymerase chain reaction HIV
2009/10/20
Oral hairy leukoplakia (OHL) is a lesion associated with a compromised immune system, and its diagnosis is determined by the demonstration of the presence of Epstein-Barr virus (EBV) in lesional tissu...
Analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (PCR)
Actinobacillus actinomycetemcomitans Porphyromonas gingivalis Prevotella intermedia PCR
2009/10/19
The presence of DNA of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by ...
HPV in oral squamous cell carcinomas of a Brazilian population:amplification by PCR
Carcinoma squamous cell Polymerase chain reaction Papillomavirus human Oncogenic viruses
2009/10/19
Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly c...
Global Gene Expression Analyses of Mouse Fibroblast L929 Cells Exposed to IC50 MMA by DNA Microarray and Confirmation of Four Detoxification Genes' Expression by Real-time PCR
MMA cDNA microarray Gene expression
2009/9/14
Methyl methacrylate (MMA) is the main component of methyl methacrylic resin, which is widely used in dentistry. Previous studies have investigated whether MMA has any adverse effects on growth and gen...
Method for rapid identification of oral streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer gene
16S-23S rRNA intergenic spacer gene Oral streptococci PCR Species identification
2008/4/16
Mutans group streptococci play an important role in the formation of dental caries, while mitis group streptococci are considered to be associated with subacute bacterial endocarditis. Both mitis and ...
RT－PCR方法研究SHH及其受体PTC在小鼠牙胚钟状晚期的表达
2007/7/28
期刊信息
篇名
RT－PCR方法研究SHH及其受体PTC在小鼠牙胚钟状晚期的表达
语种
中文
撰写或编译
作者
周彦秋,林久祥等
第一作者单位
刊物名称
北京大学学报
页面
2002, Vol. 34(2): 13
出版日期
2002年
月
日
文章标识(ISSN)
相关项目
Shh(Sonic Hedgehog)基因在颅面发育过程中作用及其机制研究
实时定量PCR分析脂肪酸合酶在口腔鳞癌中的表达
口腔鳞状细胞癌 实时定量PCR 脂肪酸合酶
2010/8/23
目的利用实时定量BCD （ <=>BCD）相对定量脂肪酸合酶（ 89:）在口腔鳞癌、癌周以及正常口腔组织中的表达情况。方法切取口腔鳞癌患者新鲜手术标本的癌组织、癌周组织及正常组织，将组织切剪成碎块，提取细胞总D%9，经寡聚脱氧胸腺嘧啶逆转录，实时定量BCD扩增，针对内参照I9B(J基因相对定量89:的表达，比较89:在?种组织中的表达。结果89:在癌组织中高表达，
Polymorphism of LPL Locus in Japanese and Comparison of PCR Amplification Efficiency from Degraded DNA between LPL Locus and the D21S11
Short tandem repeat (STR) LPL Japanese population D21S11 Degraded DNA
2010/1/21
The short tandem repeat (STR) polymorphism of the lipoprotein lipase (LPL) locus was amplified by PCR and analyzed using denaturing polyacrylamide gel electrophoresis followed by silver staining. Amon...
Polymorphism of LPL Locus in Japanese and Comparison of PCR Amplification Efficiency from Degraded DNA between LPL Locus and the D21S11
Short tandem repeat LPL Japanese population
2010/12/1
The short tandem repeat (STR) polymorphism of the lipoprotein lipase (LPL) locus was amplified by PCR and analyzed using denaturing polyacrylamide gel electrophoresis followed by silver staining. Amon...
AP-PCR基因指纹用于变形链球菌传播株与非传播株的筛选
变形链球菌 传播 随机引物—聚合酶链反应 基因型 定植
2010/8/20
目的 应用AP- PCR 基因指纹筛选变形链球菌( mutans streptococci ,MS) 的传播株(transmitted strains) 与非传播株(nontransmitted strains) ,探讨影响MS 传播的因素。方法 选取20 对口腔中已定居有MS 的儿童(3～4 岁)与他们的母亲,取牙面菌斑样本涂于轻唾—杆菌肽培养基。每人随机挑取45 株分离株,提取染色体DNA ,...
PCR和核酸探针在临床尖周炎检测中应用的对比研究
PCR 核酸探针 细菌学检测 牙龈卟啉菌
2010/9/14
采用聚合酶链反应(PCR ) 和核酸探针杂交技术, 检测了100 例临床尖周炎患牙根管内标本中牙龈卟啉菌(Pg) 的分布, 并对两种方法进行了对比研究。结果显示: ①PCR 对尖周炎中Pg 的检出率为74% , 核酸探针为76% ,两者的总符合率达到94%; ②在两种方法检测的所有项目对比组之间均未见显著性差异(P > 0105) ; ③Pg 与尖周炎临床症状中的自发痛、叩痛、臭味和尖周肿胀有关(...