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Pathogenicity Variations of Susceptibility and Resistance to Leishmania major MRHO/IR/75/ER Strain in BALB/c and C57BL/6 mice
Balb/c C57bl/6 Pathogenicity Iran Leishmania major MRHO/IR/75/ER
2009/12/16
Background: To compare the pathogenicity differences in two susceptible Balb/c and resistant C57bl/6 mice infected with Leishmania major MRHO/IR/75/ER as a prevalent strain of zoonotic cutaneous leish...
C57BL/6J×129/J杂交小鼠ES细胞系的建株(英)
小鼠 胚胎干细胞 杂交
2009/8/27
目的:建立C57BL/6J×129/J杂交小鼠ES细胞系。 方法: 收集3.5 d.p.c.的囊胚,培养在预先铺有小鼠成纤维细胞(MEFs)的高糖DMEM培养液中。3-4 d后,挑出内细胞团(ICM),消化后重新种到新鲜的有MEFS培养液中。等到有典型的ES样集落长出,即传代以得到永久ES细胞系。通过分析碱性磷酸酶活性,SSEA-1,Oct-4的表达和形成畸胎瘤的能力来鉴定ES细胞的多向分化能力。...