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Hypodiploidy as a prominent attributor to chromosomal aneuploidy in transgenic rabbit embryos
chromosomal aneuploidy EGFP embryo hFVIII rabbit transgenic
2015/6/5
Transgenic animals play a vital role in basic research, agriculture and pharmaceutical industries. Rabbits have the advantage of other large laboratory species in that they have a short gestation peri...
Altered pathogenicity, immunogenicity, tissue tropism and 3'-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
vian infectious bronchitis coronavirus Pathogenicity Immunogenicity Tissue tropism Genome sequence Serial passage
2016/12/20
In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of ...
Effect of Estradiol and Nonylphenol on mRNA Levels of VitellogeninII in the Liver of Chicken Embryos
nonylphenol estradiol vitellogenin II chicken embryo RT-PCR
2009/8/3
Nonylphenol is one of the endocrine disrupting agents with estrogenic activity in some vertebrates. The present study was conducted to assess estrogenic activity of p-nonylphenol (NP) in chicken embry...
Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β,and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos
p-nonylphenol ERα ERβ P450arom endocrine disruptor
2009/7/31
Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stag...
Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apoVLDL)II in the Liver of Quail Embryos
endocrine disrupters VTG II apoVLDLII quail RT-PCR
2009/7/31
The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density l...
Simplified DNA Extraction Methods for Sexing Chick Embryos
DNA extraction sexing PCR XhoI repetitive sequence embryo chicken
2009/7/31
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples ...
Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos
endocrine disrupting effects estrogens Japanese quail embryo ovotestis screening model
2009/7/31
Sex reversal effects of 17 beta-estradiol (E2), diethylstilbestrol (DES) and ethynylestradiol (EE2) on male gonads in F1 (AWE×WE) Japanese quail (Corturnix japonica) embryos were comparatively evaluat...
Glucocorticoid-Inducible Glutamine Synthetase in GH Cells of Chick Embryos,Gallus domesticus:Ontogeny of Glutamine Synthetase,GH,and Pit-1 Protein in the Pituitary Gland
corticosterone glutamine synthetase growth hormone pit-1 pituitary gland
2009/7/30
It has been known that glutamine synthetase (GS, L-glutamate ammonia ligase) is an enzyme that catalyzes ATP-dependent condensation of glutamate and ammonia to form glutamine, and that GS and growth ...
Restriction of Germline Proliferation by Soft X-ray Irradiation of Chicken Embryos and its Application to Chimera Production
blastoderm chimera embryo culture primordial germ cells soft x-ray
2009/7/30
Primordial germ cells (PGCs) are the progenitor cells of gametes. Avian PGCs are located in the central region of the area pellucida at the blastoderm stage. PGCs enter the circulation soon after the ...
Effects of Aromatase Inhibitor (Fadrozole)-Induced Sex-Reversal on Gonadal Differentiation and mRNA Expression of P450arom,AMH and ERα in Embryos and Growth in Posthatching Quail
aromatase inhibitor P450arom mRNA quail gonad sex-reversal
2009/7/30
The present study was conducted to investigate (1) the effective day of treatment with Fadrozole, nonsteroidal aromatase inhibitor (AI), during incubation for sex reversal, (2) the mRNA expression of ...
A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos
alkaline chicken DNA extraction feather sexing
2009/7/29
The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alka...
The Effect of Subacute Doses of Organophosphorus Pesticide, Nuvacron, on the Biochemical and Cytogenetic Parameters of Mice and Their Embryos
Organophosphorus genotoxicity nucleic acids enzymes mice embryo
2010/11/8
In the present work, male mice, pregnant females and their embryos were used. The animals were
orally administered with three pesticide doses (0.25, 0.3 and 0.65 mg/kg) of Nuvacron daily (for four da...