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A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken. When PGCs were irradiated with ...
Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the large size of their yolk structure. Cryopreservation of primordial...
The generation of germline chimeras by the transfer of primordial germ cells (PGCs) requires incorporation of the PGCs of the donor into the gonadal tissue of the recipient embryo. We investigated the...
猪原始生殖嵴细胞(PGCs)建系因素的研究。
对发育至第19期和第28期鸡胚性腺原始生殖细胞(primordial germ cells ,PGCs),用六种玻璃化冷冻液I:10%DMSO+10%EG+10%PVP,II:20%EG+10%PVP,III:20%DMSO+10%PVP,IV:10%DMSO+10%EG+0.5mol/LSurcose,V:20%EG+0.5mol/LSurcose,VI:20%DMSO+0.5mol/LSurc...

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