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Studies on Culture of Cells and Tissues of Crop Plants:III. Isolation of rice mesophyll protoplasts with a new cell wall lytic enzyme "funcelase"
Cell wall Cellulase "Onozuka" R-10 Funcelase Mesophyll Oryza sativa L
2009/8/6
Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...