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Antibacterial Activity of Recombinant Pig Intestinal Parasite Cecropin P4 Peptide Secreted from Pichia pastoris
Cecropin Ascars suum Pichia pastoris Methanol Antimicrobial Activity
2016/5/16
Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic,...
为了利用Cecropin B（CB）抗菌肽基因提高柑橘对溃疡病的抗性，人工合成了两个含有信号肽的Cecropin B 抗菌肽基因PR1aCB 和AATCB。洋葱表皮瞬时表达分析表明，与非分泌型CB 抗菌肽相比，PR1aCB 和AATCB 抗菌肽在细胞间隙中优势积累。进一步构建了CaMV 35S 调控 PR1aCB 和AATCB基因的植物表达载体，转化塔罗科血橙（Citrus sinensis Os...