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Computational Mining and Survey of Simple Sequence Repeats (SSRs) in Expressed Sequence Tags (ESTs) of Dicotyledonous Plants
computational mining simple sequence repeats Expressed Sequence Tags
2015/1/9
DNA markers have revolutionized the field of genetics by increasing the pace of genetic analysis. Simple sequence repeats (SSRs) are repetitions of nucleotide motifs of 1 to 5 bases and are currently ...
本项研究以雌性草鱼肠道为实验材料,采用非均一化的Oligo—dT引物定向克隆技术构建了草鱼肠道组织的
cDNA文库,对文库质量的分析结果表明:cDNA文库的库容量至少为2.3×lo5,重组率达95% ,平均插入片断长度
大于1000bp。挑取cDNA克隆进行5’端测序,总共进行了1571个成功反应,其中l4ll条ESTs长度大于100bp,初步
拼接得到939个单基因簇(Unigene),其...