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Culture of Chicken Embryos Obtained from the Anterior Region of the Magnum of the Oviduct after Removing a Thin Layer of Dense Albumen Capsule from the Ovum
chicken embryo culture dense albumen embryo manipulation fertilised ovum
2009/7/31
Fertilised ova, obtained from the anterior region of the magnum of the oviduct 60-80min after the preceding egg had been laid, were cultured in vitro with dense albumen adjusted to pH 7.0 after removi...
Effect of Spermidine on Intracellular Calcium Ion Mobilization in Chicken Phagocytes Treated with Leukotriene B4 (LTB4)
spermidine LTB4 calcium ion chicken phagocytes
2009/7/31
The effect of spermidine, a type of polyamine present in exudates from inflamed tissues and bacteria-infected sites in humans, on calcium mobilization of leukotriene B4 (LTB4)-stimulated chicken abdom...
Confocal Laser Scanning Microscopy on the Distribution of Neural Nitric Oxide Synthase (nNOS)-immunoreactive Nerves in the Chicken Harderian Gland
chicken confocal laser scanning microscope Harderian gland immunohistochemistry nNOS
2009/7/31
Nitrergic innervation of the chicken Harderian gland was investigated by using immunohistochemistry for neural nitric oxide synthase (nNOS) and a confocal laser scanning microscope. Immunoreactivity f...
Intracellular Calcium Ion Elevation in Chicken Phagocytes Treated with Activating Agents
intracellular calcium ion chicken macrophage heterophil
2009/7/31
Cytoplasmic calcium ion concentrations [Ca2+]i were measured for the first time in chicken heterophils and macrophages stimulated with Leukotriene B4 (LTB4), phorbol myristate acetate (PMA), formyl-me...
Effects of Soft X-Ray Irradiation to Stage X Blastoderm on Restriction of Proliferation of Primordial Germ Cells in Early Chicken Embryos
chick embryo primordial germ cell soft X-ray stage X blastoderm
2009/7/30
It is necessary to deplete the endogenous primordial germ cells (PGCs) from recipient embryos when germline chimaeric chickens are produced by the transfer of PGCs. In this study, effects of soft X-ra...
Effect of T-2 Toxin in Combination with Vitamin E,Selenium and Mycotoxin Binder on Lipid Peroxide Status and on the Glutathione Redox System in Broiler Chicken
glutathione glutathione-peroxidase lipid peroxidation T-2 toxin vitamin C
2009/7/30
The effect of T-2 toxin (2.35mg/kg of feed) on lipid peroxidation and on the antioxidant defence system was studied in blood plasma, red blood cells (RBC), liver and spleen of broiler chickens. The in...
Influences of MHC Class II Haplotypes on Avian Influenza Traits in Thai Indigenous Chicken
avian influenza virus major histocompatibility complex thai indigenous chicken
2009/7/30
Natural infections with influenza viruses have been reported in a variety of animal species including human, pigs, horses, sea mammals, mustelids and birds. Occasionally devastating pandemics occur in...
Newcastle Disease in Vaccinated Commercial Broiler Chicken Flocks Experiencing Subclinical Infectious Bursal Disease in Jordan
Technical Department Provimi-Jordan Jordan
2009/7/30
important viruses in poultry, causing catastrophic economical losses to farmers and to chicken breeding companies in Jordan. Fifteen commercial broiler chicken flocks were vaccinated twice against NDV...
Mitochondrial Oxidative Damage in Chicken Skeletal Muscle Induced by Acute Heat Stress
2D electrophoresis-protein carbonyl heat stress lipid peroxidation MDA oxidized protein
2009/7/30
Mitochondria have long been known to play a critical role in maintaining bioenergetic status under physiological conditions. Our previous studies have shown that acute heat stress increases production...
Preparation and Application for Immunocytochemistry of Antibody to Gallinacin-3,an Antimicrobial Peptide,in Chicken
antibody preparation antimicrobial peptide chicken gallinacin
2009/7/30
The aim of this study was to develop an antibody to gallinacin-3 which is one of the antimicrobial peptides in chickens. The antibody was raised in rabbit using a synthetic peptide of gallinacin-3 (Ga...
Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo
gonads green fluorescent protein primordial germ cell transfection
2009/7/30
The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA efficiently in the gonads of developing chicke...
Influence of Leucine on Protein Metabolism of Chicken Embryo Myoblasts is Modulated by Varying Serum Concentrations in the Medium
fetal calf serum leucine myoblast protein degradation protein synthesis
2009/7/30
The influence of varying leucine and fetal calf serum (FCS) concentrations in the culture medium on protein synthesis and degradation of chicken embryo myoblasts was examined. Medium199 contained 0, 1...
Structure and Tissue Distribution of Cholecystokinin-1 Receptor in Chicken
cDNA cloning chicken cholecystokinin receptor
2009/7/30
Chicken cholecystokinin-1 receptor (chCCK1R) cDNA was isolated from a chicken brain and tissue distribution of the chCCK1R mRNA was determined by RT-PCR. The chCCK1R is composed of 429 amino acid resi...
Dynamic Analysis of the Developmental Fate of Cells in the Center of the Area Pellucida of the Blastoderm in Chicken
migration PKH26 primordial germ cells vasa
2009/7/30
The cells in the center of the area pellucida of the blastoderm have been used as donors for production of chimeric chickens, but the developmental fate of the cells has not been analyzed in detail. A...
Stability of Chicken IgY Antibodies Freeze-Dried in the Presence of Lactose,Sucrose and Threalose
ELISA freeze-drying IgY lactose sucrose threalose
2009/7/30
Freeze-drying is used as a method to stabilize proteins. The process itself may however cause protein unfolding and denaturation, but the risk is reduced if a stabilizing agent is added prior to free...