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Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Effect of Estradiol and Nonylphenol on mRNA Levels of VitellogeninII in the Liver of Chicken Embryos
nonylphenol estradiol vitellogenin II chicken embryo RT-PCR
2009/8/3
Nonylphenol is one of the endocrine disrupting agents with estrogenic activity in some vertebrates. The present study was conducted to assess estrogenic activity of p-nonylphenol (NP) in chicken embry...
Organ-specificity of Estrogen Effects on the Induction of Immunocompetent Cells in the Chicken
diethylstilbestrol immunocompetent cells organ-specificity chicken
2009/8/3
Injection of immature hens with estrogen increases the population of antigen-presenting cells, T cells and Ig-containing cells in the ovary and oviduct. The aim of this study was to determine the org...
Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β,and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos
p-nonylphenol ERα ERβ P450arom endocrine disruptor
2009/7/31
Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stag...
Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
Review on Chicken Intestinal Villus Histological Alterations Related with Intestinal Function
Chicken Villus Alterations
2009/7/31
Review on Chicken Intestinal Villus Histological Alterations Related with Intestinal Function.
The present experiment was designed to investigate a possibility of the in vitro cultured somatic cells to produce the germ line interspecific chimera in bird. The frozen-thawed fibroblast-like cells ...
A Simple Method for Sexing Chicken Embryos at Stage X
blastodermal cell chicken embryo embryo culture PCR sexing
2009/7/31
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by...
Preparation of Monoclonal Antibody Against Chicken Apolipoprotein B and Development of Enzyme Liked Immunosolvent Assay (ELISA) Method with the Antibody Aiming at the Optimization of Lipid Metabolism in Chickens
apolipoprotein B monoclonal antibody enzyme linked immunosorbent assay fat deposition broiler chickens
2009/7/31
Apolipoprotein B (apoB) containing lipoproteins are major lipoproteins in blood and the elevated concentration in plasma may lead to increased transport of lipoproteins into adipose tissues resulting ...
Comparative Study on the Distribution of Glucagon-like Peptide-1 (GLP-1)-immunoreactive Cells in the Intestine of Chicken and Ostrich
glucagon-like peptide-1 immunohistochemistry intestine chicken ostrich
2009/7/31
The distribution of glucagon-like peptide-1 (GLP-1)-immunoreactive cells was investigated in the intestine of the chicken and ostrich. GLP-1-immunoreactive endocrine cells were distributed only in the...
Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
blastoderm electrode electroporation GFP gene
2009/7/31
In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (...
Effects of Semi-purified Pellet Diet on the Chicken Intestinal Villus Histology
semi-purified diet pellet intestinal villi absorptive epithelial cells histological change
2009/7/31
To establish the basic histological data on intestinal villi in chickens fed a semi-purified pellet diet, birds were divided into the following groups : intact control (IC), 3-d fasting (F), ad libitu...
Changes in Calcium, Magnesium and Phosphorus Contents of Eggshell during Stay in Oviduct Uterus in the Guineafowl and the Chicken
guineafowl chicken eggshell shell calcium shell magnesium shell phosphorus
2009/7/31
The amount of calcium, magnesium and phosphorus in the shell portion (shell with shell membrane) of eggs of guineafowl and chicken at various times during the stay in the uterus was measured by an ato...
Dioxin Concentrations in Body Tissues and Egg of Female Chicken
accumulation body tissues chicken dioxins egg
2009/7/31
To investigate the transfer and accumulation of ingested dioxins, the concentra-tions of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and coplanar-polychlorinated ...
Immunohistochemical and Morphometrical Studies on the Distribution of Glucagon-like Peptide-1 (GLP-1)-immunoreactive Cells in the Chicken Intestine
chicken glucagon-like peptide-1 immunohistochemistry intestine morphometry
2009/7/31
The distribution of glucagon-like peptide-1 (GLP-1)-containing endocrine cells was investigated in the chicken intestine by using imunohistochemical and morphometrical techniques. GLP-1-immunoreactive...