搜索结果: 31-45 共查到“农学 microsatellite”相关记录47条 . 查询时间(0.078 秒)
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
不同猪种肠毒素大肠杆菌（ETEC）F4受体微卫星标记遗传差异性研究The Genetic Variation of Two Microsatellite Markers of Escherichia coli F4(K88) Receptor in Different Swine Breeds
ETEC 微卫星 基因杂合度 Shannon信息指数 遗传标记
2008/1/10
摘要采用13号染色体上与K88ab和K88ac受体基因连锁的2对引物（S0223和S0068），研究沙子岭猪和大约克猪的遗传差异性。结果表明，2个猪种在2个基因座均存在多态性，其基因杂合度和Shannon信息指数存在很大差异，而中外猪种的K88ab和K88ac受体基因也存在遗传差异，这2对引物可望作为K88ab和K88ac受体基因的遗传标记。Abstract: The genetic variat...
利用微卫星标记进行凉山半细毛羊亲权鉴定的研究Microsatellite Markers for Paternity Testing of Liangshan Semi-fine Wool Sheep
凉山半细毛羊 微卫星标记 多重PCR 亲权鉴定
2008/1/6
摘要
经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物，并用Cervus 2.0分析。结果表明：当双亲未知时，累积排除概率为0.998666 ，在父系半同胞基础上进行母子亲权鉴定，累积排除概率为0.999994，置信度为95%，最终从8个候选父亲中找出215个后裔的父亲，又从147个候选母亲中找出它们的母亲，构建了一个混合家系系谱，为进行连锁分析、QTL定位...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC)；结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...
小麦SSR标记的发展及应用Microsatellite Marker Development and Applications in Wheat Genetics and Breeding
小麦 微卫星 分子标记 遗传图谱 遗传多样性
2007/12/30
摘要微卫星是以1～6个碱基为基本单元的串联重复序列，由于具有共显性、多态性高和容易用PCR方法检测等特点，是非常有用的遗传标记。在小麦中，SSR标记已广泛应用于遗传图谱的构建、遗传多样性、品种及基因型鉴定、目的基因，以及QTL的标记和标记辅助选择育种。
Abstract:Microsatellites are simple,tandemly repeated one to six nucleot...
Genetic Relationship among the Korean Native and Alien Horses Estimated by Microsatellite Polymorphism
Genetic Relationship Korean Native Horse Microsatellite Polymorphism
2016/4/21
Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean na...
Molecular Characterization of Hallikar Breed of Cattle Using Microsatellite Markers
Hallikar Draft Cattle Microsatellite Heterozygosity
2016/4/21
Molecular characterization of Hallikar, the native cattle breed of Karnataka, was undertaken using 19 cattle specific, highly polymorphic microsatellite markers recommended by FAO. The genomic DNA was...
FINE MAPPING OF MILK YIELD QTL ON CHROMOSOMES 6 AND 20 IN GERMAN HOLSTEIN POPULATION USING MICROSATELLITE MARKERS
production traits microsatellite markers association analysis
2010/12/1
In German Holstein population a very signifi cant QTL with effects on milk yield trait (MY) was mapped on chromosome 6 and 20 to the interval BM143–ILSTS97 and AGLA29–ILSTS72, respectively. Where, thr...
Implications for the use of horse hair roots as a DNA source for microsatellite typing
hair follicles short tandem repeats multiplex PCR
2015/6/1
Hair roots are a very attractive source of DNA for microsatellite-based parentage control of breeding animals. However, unlike blood samples, irregular DNA typing results have been observed in assays ...
Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species
Microsatellites Cross-species Amplification Mystus nemurus Pangasius micronemus Clarias batrachus
2016/4/20
The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other tha...
Population Structure and Genetic Bottleneck Analysis of Ankleshwar Poultry Breed by Microsatellite Markers
Ankleshwar Bottleneck Genetic Diversity Microsatellites Poultry
2016/4/19
Genetic variation at 25 microsatellite loci, population structure, and genetic bottleneck hypothesis were examined for Ankleshwar poultry population found in Gujrat, India. The estimates of genetic va...
Establishment of an Individual Identification System Based on Microsatellite Polymorphisms in Korean Cattle (Hanwoo)
Individual Identification Microsatellites Polymorphic Information Content Cattle
2016/4/19
This study was conducted to establish an individual identification system comprising of 19 microsatellite markers located on different bovine autosomes. The markers were typed on 257 animals from five...
Microsatellite DNA Typing Using 16 Markers for Parentage Verification of the Korean Native Horse
Allele Korean Native Horse Microsatellite DNA Parentage Verification
2016/4/15
This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite marker...
Evaluation of the Genetic Relationship among Ten Chinese Indigenous Pig Breeds with Twenty-six Microsatellite Markers
Microsatellites Chinese Indigenous Pig Breeds Genetic Variation
2016/4/15
The genetic diversities and relationships of 10 Chinese indigenous pig breeds and three exotic pig breeds have been evaluated using 26 microsatellites recommended by the Food and Agriculture Organizat...
Association Analysis between Five Microsatellite Loci and Litter Size in Small Tail Han Sheep
Sheep Litter Size Microsatellite Polymorphism Least Squares Analysis
2016/4/14
The objective of the present study was to explore associations between five microsatellites linked to FecB and FecXI genes and litter size in Small Tail Han sheep. The polymorphisms of five microsatel...