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铜绿假单胞菌和大肠杆菌是医院和兽医临床上常见的病原菌，容易造成人类和动物感染，给疾病治疗和经济发展带来极大的挑战。为了开发出靶向铜绿假单胞菌和大肠杆菌黏肽合成酶（PBPs）的新型先导化合物，笔者以铜绿假单胞菌PBP3和大肠杆菌PBP1b、PBP4、PBP5作为靶点，针对ZINC数据库，使用分子对接软件DOCK6.5进行了虚拟筛选。之后，根据得分情况和结构分析，找出了具有全新结构的先导化合物，进行了...
Dietary Energy Level Affects the Composition of Cecal Microbiota of Starter Pekin Ducklings
duck bacterial diversity dietary energy
2018/3/5
In this study, we evaluated the phylogenetic diversity of the cecal microbiota of 3-week-old ducklings fed three
diets differing in metabolizable energy. The contents of the ceca were collected from ...
Effects of diet supplementation with herbal blend and sunflower seeds on fermentation parameters,microbial population,and fatty acid profile in rumen of sheep
16S-PCR-DGGE medicinal herbs phagocytic activity lipid metabolism rumen fluid PUFA source
2016/12/20
The study evaluated the rumen fermentation pattern, microbial population, fatty acid composition, and antioxidant status in the rumen fluid and blood of sheep in response to a diet supplemented with h...
Antimicrobial activity of lactic acid bacteria multiplied in an alternative substrate and their influence on physiological parameters of new-born calves
alternative substrate antimicrobial activity blood parameters calves faecal parameters lactic acid bacteria
2016/12/8
Here, the ability of Pediococcus pentosaceus and Pediococcus acidilactici to utilise potato tuber juice for cell synthesis without an external nutrient supplement was investigated, and the influence o...
Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey
pulsed-field gel electrophoresis virulence genes bovine rectal swabs E. coli strains
2016/12/8
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of ...
Apparent total tract digestibility and ileal digestibility of dry matter,nitrogen,energy and amino acids in conventional,Bacillus subtilis-fermented and enzyme-treated soybean meal fed to weanling pigs
growth performance fermented soybean meal anti-nutritional factors antigens lectins trypsin inhibitors Bacillus subtilis T-cannula surgery
2016/12/8
Apparent total tract digestibility (ATTD), apparent ileal digestibility (AID) and standardised ileal digestibility (SID) of dry matter, nitrogen, energy and amino acids were evaluated in six weanling ...
Fluorescence microscopy methods for the determination of somatic cell count in raw cow’s milk
microscopy cow milk fluorescence flow cytometry temperature somatic cell wall temperature DMSCC
2016/12/8
The direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow’s milk. However, this method has some limitations that may influence the resul...
Antidermatophyte and antioxidant activities of Nigella sativa alone and in combination with enilconazole in treatment of dermatophytosis in cattle
Nigella sativa enilconazole antioxidants trichophytosis bovine
2016/11/11
The purpose of this study was to comparatively assess the antidermatophyte and antioxidant activities of enilconazole, Nigella sativa (NS) and enilconazole with NS in the treatment of dermatophytosis ...
Effect of linseed and the combination of conjugated linoleic acid and linseed on the quality and oxidative stability of pig meat and subcutaneous fat
CLA meat quality backfat TBARS fatty acids
2016/9/1
The aim of this experiment was to test the hypothesis that conjugated linoleic acid (CLA) in diets of finishing pigs fed linseed can improve the quality and oxidative stability of meat and subcutaneou...
Freezing and storage of leukodepleted erythrocyte suspensions
canine cryopreservation leukocyte quality red blood cell
2016/9/1
Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet be...
Presence of Arcobacter species in pet cats and dogs in the Czech Republic
Arcobacter butzleri cat dog polymerase chain reaction oral smears
2016/9/1
This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infecti...
Production value and cost-effectiveness of pig fattening using liquid feeding or enzyme-supplemented dry mixes containing rye grain
swine barley fattener enzyme yeast xylanase
2016/9/1
The aim of this study was to assess the cost-effectiveness of a fermented liquid feeding and enzyme supplementation of dry fodders containing rye grain for pig fattening. Two experiments were performe...
Effect of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on faecal microbiota composition in weaned piglets
dietary supplements humic substances zinc bacterial community composition
2016/7/6
The aim of this study was to use high throughput sequencing of piglet faeces to investigate if the favourable effects of sodium humate (HNa) and zinc oxide (ZnO) in post-weaning diarrhoea (PWD) treatm...
Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions
phagocytic chemotactic activity cortisol IL-8 CXCR1 selectin integrin
2016/6/3
The goal of this work was to understand how polymorphonuclear leukocytes (PMN), the first line of cellular defence, integrate and prioritize multiple chemotactic signals to navigate during periods of ...