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猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和猪轮状病毒(PoRV)是三种常见的仔猪病毒性腹泻病原，作者拟构建可同时检测三种病原的cDNA基因芯片。分别根据PEDV基因（S、M）、TGEV基因（S、N）、PoRV基因（VP7、NSP4）的保守区设计引物扩增靶基因，进行PEDV-TGEV-PoRV的cDNA阵列设计，建立了扩增标记探针的多重PCR方法，并对所构建cDNA芯片的特异性、...
旨在探究1型鸭甲肝病毒（DHAV-1）VP3蛋白抗血清的中和活性并鉴定VP3的线性B细胞表位。利用pGEX-4T-1表达载体，在BL21(DE3)宿主菌中原核表达DHAV-1 VP3基因，以切胶纯化出的蛋白质为抗原免疫兔制备多克隆抗体，通过鸡胚中和试验对多抗的中和效价进行检测；采用Karplus&Schulz、Emini、Jameson-Wolf和Parker方法分别对柔韧性、表面可及性、抗原性及...
Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection
chicken infectious anaemia virus cytokine real time-PCR viral load antibody titre immunosuppression
2015/4/17
The present work was carried out to investigate the correlation between viral load and the cytokine profile and virus specific antibody levels at five day intervals up to 25 days post infection (dpi) ...
Fatal canine adenovirus type 1 acute infection in a Yorkshire Terrier puppy in Portugal: a case report
canine adenovirus type 1 CAdV-1 infectious canine hepatitis Mastadenovirus
2015/4/17
This study reports the diagnostic algorithm followed for the identification of a fatal canine adenovirus type 1 (CAdV-1) infection in an unvaccinated 56 day-old puppy to overcome the limitations impos...
Selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia
goat serum glucose β-hydroxybutyrate cytokines enzymes minerals aciduria ketonuria acute phase proteins
2015/4/17
The purpose of this study was to examine selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia. Seventy five female goats w...
Chocolate ingestion-induced non-cardiogenic pulmonary oedema in a puppy: a case report
chocolate intoxication non-cardiogenic pulmonary oedema methylxanthines furosemide
2015/4/17
Chocolate intoxication in small animals may be life-threatening and associated with serious morbidity and mortality. The main clinical presentations are cardio-respiratory and neurological complicatio...
Animal welfare of barrows with different antemortem lairage times without food
rest hemodynamic response stress pig lactate
2015/4/17
This study evaluated the effect of five different periods of antemortem lairage without food on the energy metabolism, gas exchange, mineral and blood acid-base balances of 1174 hybrid barrows, which ...
Detection of Visna Maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection
Visna/Maedi virus sheep lymphoid tissue immunohistochemistry PCR experimental infection
2015/4/16
Visna/Maedi virus (VMV), a small ruminant lentivirus responsible for lymphoproliferative pneumonia, encephalitis, arthritis and/or mastitis in sheep, has been detected in different non-lymphoid organs...
O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea
Escherichia coli piglet O-serogroup pathotype virulence gene
2015/4/16
A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin,...
A review on Schmallenberg virus infection: a newly emerging disease of cattle, sheep and goats
abortion congenital malformation emerging infection pathology pathogenesis ruminants Schmallenberg virus stillbirths
2015/4/16
Schmallenberg virus (SBV) infection is an emerging infectious disease of ruminants first described in Germany in November, 2011. Since then it has spread very rapidly to several European countries. Th...
Co-infection of bovine papillomavirus type-1 and-10 in teat warts of dairy cattle
teat wart bovine papillomavirus cattle, PCR real time PCR
2015/4/16
The present study was carried out to investigate the involvement of different bovine papillomaviruses in the teat warts of cattle. A total of 11 teat wart samples showing rice grain-like and small, se...
Descriptive study of botulism in an Austrian dairy herd: a case report
botulism herd case flaccid tongue recumbency mouse bioassay
2015/4/16
An outbreak of botulism was suspected at an Austrian dairy farm in June 2010. Six Simmental cows, out of a herd of 29, were affected and showed the typical signs. The affected cows included either ani...
Prevalence of etiological agents of selected respiratory infections in chicken and turkey farms in the Czech Republic
respiratory pathogens turkey polymerase chain reaction
2015/4/16
The causative agents of respiratory diseases of turkeys represent, primarily in fattening farms, a substantial risk of economic and breeding problems. The purpose of this communication is to provide i...
The prevalence of hepatitis E virus in piglets on Czech pig production farms and phylogenetic analysis of recovered isolates
swine hepatitis E virus real time RT-PCR nested RT-PCR genotype 3
2015/4/16
The aim of our study was to determine the prevalence of hepatitis E virus (HEV) in domestic pigs and to investigate the genetic divergence of swine HEV in the Czech Republic. To this end, a one-step r...
Mycobacterium avium subsp. paratuberculosis in powdered infant milk: F57 competitive real time PCR
Crohn's disease public health food safety environmental risk
2015/4/15
Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More t...