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Lead induced alterations in rabbit spermatozoa motility and morphology in vitro
reproductive toxicity rabbit Pb temperature sperm motility morphological abnormality
2016/11/11
The aim of this in vitro study was to determine the effect of lead chloride (PbCl2) on rabbit spermatozoa motility and morphology. Lead concentrations in the medium ranged between 0.45 and 11.17 μg/ml...
本研究旨在通过构建腺病毒介导的体外超表达载体，探究腺苷甲硫氨酸转移酶（MAT2B）对猪肌内脂肪细胞分化的影响。本研究以猪脂肪组织为试验材料，提取总RNA，并反转录得到cDNA，参考GenBank收录的猪MAT2B基因mRNA序列设计引物，PCR扩增并连接到腺病毒穿梭载体pAdTrack-CMV，进行测序鉴定。结果表明，构建的穿梭载体与骨架载体pAdEasy-1可以实现同源重组，即腺病毒重组载体pA...
为明确大豆品种对大豆食心虫成虫产卵选择的抗性机制，以4 个不同抗性水平的大豆品种（系）为材料进行大豆食心虫成虫产卵选择性试验，研究分析豆荚株位、豆荚大小、荚毛长度、荚毛密度、荚毛颜色等特征性状与成虫着卵量的关系。结果表明，在相同环境条件下，品种间着卵量存在显著差异，在豆株上大豆食心虫成虫喜于中、下部豆荚上产卵；着卵量与豆荚大小呈显著正相关，模型为y荚长= 3.16421 + EXP( 33.271...
Successful response to imatinib in two dogs with inoperable grade III infiltrating mast cell tumours:a case report
canine KIT oncogene target therapy tyrosine kinase inhibitor
2016/9/1
Two dogs were presented owing to sudden rapid growth of cutaneous masses greater than 10 cm on the neck and axillary region, respectively. Based on history and results of physical examinations, blood ...
金黄色葡萄球菌对奶牛乳腺成纤维细胞TGF-β1/Smad信号通路及其转分化的影响
金黄色葡萄球菌 奶牛乳腺成纤维细胞 &alpha -SMA collagen-Ⅰ p-Smad2/3
2016/7/26
为探索金黄色葡萄球菌（S.aureus）对奶牛乳腺成纤维细胞(BMFB)TGF-β1/Smad信号通路及其转分化的影响，用热灭活金黄色葡萄球菌（0、10 4，10 5，10 6，10 7和10 8 CFU•mL-1）刺激BMFB，24 h后采用Real-time PCR 方法检测TGF-β1、α-SMA和collagen-Ⅰ mRNA的转录量，Western blot方法检测α-SMA...
Evaluation of early renal disease in bitches with pyometra based on renal doppler measurements
renal doppler ultrasonography pyometra dog
2016/7/6
Pyometra is a widely detected, chronic infection of the uterus. One of the complications of this infection is renal disease. Kidney biopsy, urinary biomarkers and blood tests were done to detect pyome...
A challenging radiographic diagnosis–extraluminal leiomyoma of the oesophagus in a small-sized dog:a case report
dog oesophagus leiomyoma oesophagram mediastinal mass
2016/7/6
An 11-year-old, intact male Maltese dog presented with a history of vomiting and regurgitation. On plain radiographs, a caudodorsal thoracic mass was identified, and there were no radiographic signs f...
potD基因编码的蛋白质作为转运体蛋白，负责多胺的结合和转运，为细胞的正常生长提供必需因子，已在多种病原菌中鉴定为毒力相关因子，但在副猪嗜血杆菌中的作用还不清楚。利用自然转化法构建potD缺失株SC1401ΔpotD::kan，再比较亲本株和缺失株的生长特性、自凝集活性、生物被膜形成能力、抗血清杀菌能力以及对小鼠的致病力。结果显示potD基因的缺失不影响副猪嗜血杆菌的生长、自凝集活性和生物被膜形成...
旨在比较观察有钩和无钩豆状囊尾蚴的头节在相对静止和相对运动时的超微结构变化。在屠宰检验和病理剖检过程中采取发育良好的豆状囊尾蚴，直接剪破囊泡从中取出豆状囊尾蚴；或用含10％兔胆汁生理盐水培养，使囊尾蚴从囊泡中翻出，制作样品，用扫描电镜观察和记录。结果显示：有钩和无钩豆状囊尾蚴在不同状态下的超微结构差异主要出现在顶突。有钩豆状囊尾蚴在相对静止时顶突的前端比较钝，通过皮肌柱与鹿角样的小钩相连。在轻度运...
Alveolar rhabdomyosarcoma in a dog confirmed using myogenin immunohistochemistry:a case report
alveolar rhabdomyosarcoma desmin myogenin skeletal muscle vimentin
2016/6/3
A large mass from the right forelimb of a five-year-old, male, Maltese dog, was resected surgically and examined histopathologically. Grossly, the 4 × 6 cm mass was well-demarcated and firm. Microscop...
An abdominal cavity abscess associated with Salmonella enterica serovar Typhimurium phage type DT2 in a dog:a case report
abdominal abscess dog Salmonella enterica serovar Typhimurium variant DT2 minimum inhibitory concentration resistance profile
2016/6/3
Most Salmonella enterica serovar Typhimurium strains are capable of infecting multiple hosts. In this report, an abdominal cavity abscess associated with the Salmonella enterica serovar Typhimurium ph...
Septic shock associated with complex infection by crop Candida and bacteria in two blue-fronted amazon parrots: a case report
blue-fronted amazon parrot sepsis candidiasis bacterial infection histopathology diagnosis
2016/6/3
Infectious disease is frequently associated with morbidity and mortality in companion birds. The clinical features of these bacterial and fungal diseases may be influenced by stress factors and the co...
维生素C对藏猪精液4 ℃保存及其精子全基因组DNA甲基化的影响
维生素C 藏猪精液 4 ℃保存 ＤＮＡ甲基化
2016/5/23
旨在研究维生素C对藏猪精液4 ℃保存效果及其精子全基因组DNA甲基化的影响。用手握法采集健康藏猪精液，在改进的猪精液低温稀释液中添加不同浓度的维生素C（0.0、2.5、5.0、7.5、10.0 g•L-1），4 ℃保存到第5天时，以藏猪精子活力、精子畸形率、精子顶体完整率和质膜完整率为评价指标，探讨维生素C对藏猪精液4 ℃保存的效果；用甲基化荧光定量法检测藏猪新鲜精液组、保存到第5天时...
H5N1亚型禽流感病毒感染番鸭引起肺损伤和脑损伤的机制探索
禽流感病毒 H5N1亚型 调控 损伤
2016/5/24
旨在初步探讨H5N1禽流感病毒引起番鸭肺和脑组织损伤的潜在机制。采用两株H5N1禽流感病毒DK383 (A/Duck/Guangdong/383/2008) 和DK212 (A/Duck/Guangdong/212/2004)分别感染番鸭，于感染后1、2和3 d取肺、脑组织，进行病理学观察，并检测病毒复制水平及损伤相关因子的变化情况。结果表明，DK383株感染番鸭后，引起明显的呼吸困难和神经症状，...
Effects of dietary conjugated linoleic acid on the duodenal mucosal immunity response and redox status of broiler chicks infected with Eimeria aceruvlina
CLA intestinal mucosal immunity oxidative stress broiler chick coccidiosis
2016/5/11
The present experiment investigated the effects of conjugated linoleic acid (CLA) on the growth performance, oocyst shedding, duodenal mucosal immune response, and redox status of broiler chicks infec...