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Selenium metabolism in goats – maternal transfer of selenium to newborn kids
mother-young ruminants trace element
2015/4/2
The aim of our study was to compare the concentration of selenium (Se) and the activity of glutathione peroxidase (GSH-Px) in the whole blood of goats and their newborn kids. The experiment involved 2...
Postnatal morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets
gnotobiotic piglets intestinal morphology short-chain fatty acids
2015/4/2
The present study examined the impact of Enterococcus faecium on morphological development and production of short-chain fatty acids in the digestive tract of gnotobiotic piglets during milk nutrition...
Bull and boar sperm DNA integrity evaluated by sperm chromatin structure assay in the Czech Republic
flow cytometry bull boar fertility
2015/2/5
Analysis of sperm parameters is very important for predicting the outcome of assisted reproductive techniques and is necessary for determination of fertility potential of males tested for artificial i...
Influence of parenteral administration of selenium and vitamin E during pregnancy on selected metabolic parameters and colostrum quality in dairy cows at parturition
cattle immunoglobulins thyroid hormones
2015/2/5
The aim of the present work was to study the influence of different dose of parenteral administration selenium and vitamin E in dairy cows prior to parturition on selected metabolic parameters and col...
The effects of diet supplemented with sodium bicarbonate upon blood pH, blood gases and eggshell quality in laying geese
feeding bicarbonate blood laying period goose
2015/2/5
The effects of diet supplemented with sodium bicarbonate (NaHCO3) upon blood pH, blood gases and eggshell quality during the laying cycle in geese were investigated. Fourteen geese aged 2 yr old were ...
旨在探明饮水中添加不同剂量硼酸对 45 d非洲雏鸵鸟肺组织形态学的影响。1 d非洲雏鸵鸟 24 羽，随机分为 6 组，分别在饮水中添加 0（A 组，对照组）、40（B 组）、80（C 组）、160（D 组）、320（E 组）、640 mg•L-1（F 组）剂量的硼酸。饲喂至45 d 取肺组织进行石蜡包埋，切片，通过 HE 染色，MASSON 染色和TUNEL 技术进行分析。与对照组相比...
Molecular Characterization of the Pathophysiology of the Digital Laminae in Acute CarbohydrateInduced Equine Laminitis
Pathophysiology Digital Laminae Acute Carbohydrate Induced Equine Laminitis
2014/10/23
Equine laminitis is a devastating condition that results in the failure of the tissue responsible for suspending the skeleton within the hoof capsule. The digital laminae is composed of two interdigit...
Tyrosine Phosphorylation Events in Mouse Sperm Capacitation
Tyrosine Phosphorylation Events Mouse Sperm
2014/10/23
Mammalian sperm are not able to fertilize immediately upon ejaculation; they become fertilization-competent after undergoing changes in the female reproductive tract collectively termed capacitation. ...
恩诺沙星在鸡体中的排泄及其在鸡粪中的降解
恩诺沙星 鸡 排泄 降解
2013/12/11
检测了在不同给药剂量下，健康鸡服用恩诺沙星后，在不同时间粪尿中恩诺沙星及其主要代谢产物的含量变化，并对在不同光照条件下鸡粪中恩诺沙星的降解进行了研究。结果表明，健康鸡灌服恩诺沙星后，主要以恩诺沙星原形、其次以环丙沙星的形式随粪（尿）排出体外。2.5 mg/kg体重剂量组和5 mg/kg体重剂量组在给药后6 h恩诺沙星的排泄量达到最高峰；环丙沙星达到峰浓度的时间为4 h。7.5 mg/kg体重剂量组...
本研究旨在探讨热应激对奶牛乳腺上皮细胞生长、凋亡及热休克蛋白（Heat shock protein，HSP）mRNA转录的影响。本文以体外高温(42 ℃)作为热应激模型温度，38 ℃作为对照温度培养奶牛乳腺上皮细胞，并采用台盼蓝染色绘制生长曲线，细胞流式检测细胞凋亡及RT-qPCR检测HSPs mRNA转录。结果表明， 42 ℃高温处理使得奶牛乳腺上皮细胞生长停滞，细胞数量在培养的第2天已显著少于...
为研究11月份间情期水貂卵母细胞的发育规律，探索其体外培养成熟的可能性,本研究利用透射电镜对间情期美国短毛黑水貂卵巢的腔前卵泡卵母细胞的超微结构进行观察。结果发现：在原始卵泡阶段，卵母细胞线粒体的嵴较少，高尔基体可见，脂滴大小不一，皮质颗粒分散在细胞核周围；初级卵泡阶段，线粒体数量增加，高尔基体趋于典型，脂滴分布均匀，皮质颗粒在质膜附近呈单层分布；次级卵泡阶段，线粒体嵴的数量增加，高尔基体在质膜下...
妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响
营养限饲 胎儿肝脏 生长发育 抗氧化能力
2013/11/28
本试验旨在研究妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响。选择健康的蒙古绵羊42只(经同期发情受孕)，在妊娠90 d时选择6只母羊进行屠宰，其余按体重随机分配到3个组：限制组1 (0.175 MJ ME·kgw-0.75·d-1，n=14，RG1)、限制组2(0.33 MJ ME·kgw-0.75·d-1，n=12，RG2)和自由采食组(0.67 MJ ME·kgw-0.75·d...
孕激素受体在雌性山羊腹腔肠系膜前神经节的分布
腹腔肠系膜前神经节 孕激素受体 免疫组织化学SP法 雌山羊
2013/11/28
为了研究孕激素对胃肠道的内分泌调节和自主神经对胃肠道的神经调节两种途经之间的相互关系，作者采用免疫组织化学SP法，观察孕激素受体(PR)在成年雌性山羊腹腔肠系膜前神经节的分布特点。结果显示，雌山羊腹腔肠系膜前神经节中PR免疫阳性产物广泛存在，且主要分布于神经元中。神经元的细胞膜呈棕褐色，为强阳性，细胞质为强阳性或中等阳性。43.40%的神经元细胞核内核质呈黄褐色，核仁为清亮的空泡；56.60%的神...
黑素皮质素受体1（MC1R）基因与VC-獭兔毛色关系的研究
VC-獭兔 MC1R RT-PCR 毛色
2013/11/28
为研究黑素皮质素受体 (Melanocortin1 receptor，MC1R)基因对獭兔毛色的影响，本试验采用RT-PCR技术克隆了MC1R基因的cDNA片段，发现MC1R基因长951 bp，编码317个氨基酸的前体蛋白，蛋白质PI为9.26，分子量36.28 ku，具有7个跨膜结构域，与其他动物MC1R蛋白性质相符。其氨基酸序列经同源性比对结果显示：其与牦牛，马、绵羊、骆驼、牛等哺乳动物的同源...