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鸡球虫病是严重危害养鸡业的一种原虫病。长期以来本病防治主要依赖于药物，但由于耐药虫
株的频繁出现、药物残留，研制开发新药成本高、周期长等问题的存在，用免疫方法来控制鸡球虫病日益
受到重视，并有望成为主要的技术手段。文章从体液免疫和细胞免疫等方面综述了鸡球虫免疫原性，并
概述了鸡球虫免疫学的研究进展。
A field study on negative energy balance in periparturient dairy cows kept in small-holder farms: Effect on milk production
Dairy cow milk yield negative energy balance reproduction
2011/1/13
Impact of negative energy balance (NEB) and its consequences on milk production and reproduction were studied in 51 dairy cows. All cows were randomly selected from 11 small-holder farms according to ...
犬瘟热病毒核衣壳蛋白基因免疫小鼠诱导抗体的研究
犬瘟热病毒 真核表达 基因免疫
2011/4/1
将犬瘟热病毒(Canine distemper virus, CDV)核衣壳蛋白基因(Nucleocapsid protein, N)的N蛋白基
因克隆到pMD-18T载体，经酶切分析测序获得阳性重组质粒。采用EcoRⅠ和KpnⅠ双酶切后定向克隆
进真核表达载体pcDNA中，电泳测序筛选阳性克隆pcDNA-N，转染COS-7细胞，IFA验证结果显示转染
的COS-7细胞胞浆中表达了CDV的N...
猪流行性腹泻胶体金抗体检测技术的建立及其应用
猪流行性腹泻 胶体金免疫层析测定 酶联免疫吸附测定 葡萄球菌蛋白A
2011/4/2
为建立一种高效、经济、简便适用于基层防疫检疫人员使用的猪流行性腹泻(PED)疾病的检测方
法，参考已知的胶体金免疫层析法(GICA)工作原理基础之上，将葡萄球菌蛋白A(SPA)进胶体金标记作
为指示介质，将基因工程表达PEDV M蛋白抗原和自制的抗SPA多抗血清包被硝酸纤维素膜(NCM) 分
别作为检测线和质控线，由此制成一种检测PEDV血清抗体的快速诊断试纸。其特异性、敏感性试验与
EL...
为确定所分离Ⅰ型鸭肝炎病毒(DHV-Ⅰ)毒力，为DHV单克隆抗体研究做好前期准备。将Ⅰ型鸭
肝炎病毒接种于9 日龄SPF 鸡胚尿囊腔(0.2 mL/胚）进行增殖，制备鸡胚成纤维细胞(CEF），并分别运用
鸡胚半数致死量(CELD50)法和组织培养半数感染量（TCID50）法测定DHV的感染力。结果显示，DHV-Ⅰ
在SPF 鸡胚内成功增殖，致死鸡胚具有典型临床症状和体表特征，所增殖病毒的CE...
Assessment of village chicken production system and evaluation of the productive and reproductive performance of local chicken ecotype in Bure district, North west Ethiopia
Ethiopia local chicken ecotypes village chicken production system
2011/1/11
A survey was conducted in Bure district, North West Ethiopia, from 2007 to 2008 to assess the existing village chicken production system. A participatory rural appraisal and a formal survey were used ...
Effects of Dietary Addition of Bentonite on Manure Gas Emission, Health, Production, and Meat Characteristics of Hanwoo (Bos taurus coreanae) Steers
Clay Mineral Bentonite Production Meat Quality Hanwoo
2016/5/5
A study was conducted to determine the dietary effects of a clay mineral (sodium bentonite, NaB) on manure gas emission, health, production, and meat characteristics of Hanwoo steers. Two diets fed to...
Evaluation of Dry Matter Intake and Average Daily Gain Predicted by the Cornell Net Carbohydrate and Protein System in Crossbred Growing Bulls Kept in a Traditionally Confined Feeding System in China
CNCPS Model Dry Matter Intake Average Daily Gain Growing Bulls
2016/5/5
Two separate animal trials were conducted to evaluate the coincidence of dry matter intake (DMI) and average daily gain (ADG) predicted by the Cornell Net Carbohydrate and Protein System (CNCPS) and o...
Comparison of Rectal and Infrared Thermometry for Obtaining Body Temperature of Gnotobiotic Piglets in Conventional Portable Germ Free Facility
Gnotobiotic Piglet Rectal Temperature Infrared Thermometry Portable Germ Free Facility
2016/5/5
Gnotobiotic piglets (n = 10) were hand-reared in conventional germ-free facilities. Piglet body temperatures were measured with rectal and non-contact infrared thermometry (NIFT) on the lower eyelid, ...
Effect of Naked Neck Gene on Immune Competence, Serum Biochemical and Carcass Traits in Chickens under a Tropical Climate
Naked Neck Gene Immune Competence Serum Biochemical Parameters Carcass Traits Tropical Climate
2016/5/5
A comprehensive study was undertaken to evaluate the effect of naked neck (Na) gene on immune competence, serum biochemical parameters and carcass quality traits in three genotypes (NaNa, Nana and nan...
Effects of Dietary Skullcap (Scutellaria baicalensis) Extract on Laying Performance and Lipid Oxidation of Chicken Eggs
Skullcap Extract Egg Weight Malondialdehyde Haugh Unit Laying Hens
2016/5/4
This study was conducted to investigate the effect of dietary domestic Skullcap (Scutellaria baicalensis) extracts (SCE) on laying performance and egg quality in laying hens. There were no significant...
A calculation program for real-time production of ammonia gas in animal houses
Ammonia animal barn computer program
2011/1/10
Ammonia is the major air contaminant in poultry houses. Ammonia emissions from animal houses influence the atmospheric environment, since aerial ammonia is one of the so called greenhouse gases and ha...
The inhibitory effect of camel's urine on mycotoxins and fungal growth
Camel urine mycotoxins mycelial growth inhibitory effect on fungal growth
2011/1/11
The effect of urine and camel milk in the inhibition of biological effects of mycotoxins produced by nine isolates of Aspergillus flavus and one isolate of Aspergillus niger isolated from pulse seeds ...
鸡毒支原体灭活抗原超滤浓缩工艺的研究
鸡毒支原体 超滤浓缩 抗原
2009/11/10
［目的］ 建立一套适合生产鸡毒支原体灭活疫苗的抗原浓缩工艺。［方法］ 采用Millipore中型盒式超滤系统对鸡毒支原体灭活抗原进行超滤浓缩试验，并利用紫外光度法测定不同倍数浓缩滤液的吸光度变化。［结果］ 消毒液高压灭菌后无细菌污染，超滤仪回流液循环后、抗原浓缩前后均无细菌污染。瑞氏染色后发现，除尾液样品中无支原体外，其他样品中均可见形态不规则、蓝色的支原体菌体。使用100 K超滤膜堆浓缩后，支原...
Study on Forced Ventilation System of a Piglet House
computational fluid dynamics perforated ceiling piglet house
2009/9/9
The objective of this study was, using the data of field experiment and visualization of internal airflow, to clearly understand forced ventilation of a piglet house with perforated ceiling which was ...