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选取体质量约为100 kg、氟烷基因型为NN的长×大二元杂交猪60头,随机分为3组:不休息组,休息3 h组,休息3 h+玩具组,公母各半。根据评分标准分别记录休息过程中猪的精神状况。放血时收集血样,用于分析血浆中皮质醇、促肾上腺皮质激素(ACTH)浓度,乳酸脱氢酶、肌酸激酶活性,乳酸、血糖浓度;添加EDTAK2抗凝剂的血样用于血细胞分析。同时在放血时,测定血温。屠宰后,测定背最长肌24 h滴水损...
应用扫描电镜及偏振光显微镜对沙狐、狼和貉的背部和腹部直针毛的髓质指数及鳞片花纹类型进行研究。结果表明,沙狐2个部位的髓质指数区间为71.4%~82.9%,狼的为43.6%~62.9%,貉的为51.2%~66.7%;毛鳞片形态在属间和种间存在差异,同一种类身体不同部位毛的鳞片形态也有差异,同一根毛的不同部位也存在差异,沙狐背毛主要鳞片类型为方瓣型,腹毛主要鳞片类型为长瓣型;狼背毛的主要鳞片类型为方瓣...
[目的] 为探索单个转基因阳性细胞快速扩大培养成为细胞克隆的技术体系。[方法] 对转染人乳铁蛋白(Human lactoferrin,hLF)基因乳腺特异性表达载体pBLMC1的单个山羊胎儿成纤维细胞(Fetal Fibroblasts,FF)和乳腺上皮细胞(Mammary Gland Epithelial,MGE)细胞进行克隆。在96孔板中,首先用3种浓度(V/V: 0 、50%和100%...
[目的]区分成型的毛皮产品,避免假毛皮以假乱真。[方法]用哈氏切片法快速制取高清晰度毛纤维横切片,采用生物显微镜和扫描电镜观察蓝狐、水貂、獭兔3种动物的毛纤维结构。[结果]獭兔毛鳞片排列紧密,髓质发达,横切面呈花生豆状;黑貂绒很细,有髓,且鳞片翘角非常大;蓝狐针毛从根部到梢部由连续状髓过渡为断续状髓到毛尖时无髓,横切面呈椭圆形,其绒毛基本呈圆形,有髓。[结论]蓝狐、水貂、獭兔3种动物的毛纤维的结构...
莆田黑鸭体型性状的主成分分析。
【目的】研究湖羊卵巢组织BMP2、BMP4、BMP6和BMP7基因mRNA表达水平与排卵数的相关性,筛选影响湖羊多胎性状的候选基因,为揭示湖羊高繁多胎分子遗传机理提供参考。【方法】选取16只经产湖羊母羊,分为产单羔组和多羔组,发情后24~36 h屠宰,取卵巢,计数排卵点,记录排卵数;应用RT-PCR技术检测BMP2、BMP4、BMP6和BMP7基因的组织表达特征,进一步利用实时荧光定量PCR技术分...
【目的】分析圈养雌性马麝(Moschus sifanicus)的发情交配时间格局,探查性经历、年龄、来源及繁殖成效等因素对发情交配的周期数、间情期的影响,为麝类成功驯养及异地保护提供参考。【方法】采用随机取样法和所有事件记录的行为取样方法,观察马麝的交配行为,准确记录发情交配的启动和结束时刻。【结果】兴隆山麝场圈养雌麝的间情期为(12.23±0.52)d(n=22),平均动情次数为(1.88±0....
Ghrelin, a novel growth hormone (GH)-releasing acylated peptide, is recently isolated from rat stomach. This peptide stimulates the release of GH from the anterior pituitary through the GH secretagogu...
We investigated the central effect of the estrogenic pollutant, bisphenol A (BPA), on behaviors, growth and several organ weights in chicks. Four-day-old male chicks were injected into the brain with...
Using the fluorescent polarization (P) it is shown that the avian cell membranes change their fluidity in some circumstances like the mammalian cells.The cells from a liver tumor (myelocytoblastoma) w...
Octopamine, an important neurotransmitter in invertebrates, has noted several similarities with noradrenaline (NA) in mammals. To compare the effect of octopamine and NA in the central nervous system,...
Single Comb White Leghorn laying hens (Hyline) were fed ad libitum in order to estimate energy expenditure of behavioral activities (BA) such as standing, eating or body movements et cetera under arti...
This study was carried out to investigate the behavior and physiological conditions of hens housed in cages with or without perches. The physiological parameters investigated were heart rate (HR), bod...
Quinine is known as a bitter compound for humans and also used in animal experiment. However, there is no evidence that animals share the same taste sensation of quinine with humans. The purpose of th...
Seasonal changes occur in the plumage of male mallard ducks, and this phenomenon is referred to as seasonal dimorphism of the plumage. However, no information is available, especially morphological, a...

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