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Automatic Clustering of Flow Cytometry Data with Density-Based Merging
Density-Based Merging Flow Cytometry Data
2015/8/20
The ability of flow cytometry to allow fast single cell interrogation of a large number of cells has made this technology ubiquitous
and indispensable in the clinical and laboratory setting. A ...
Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx
bovine virgin mammary gland induced influx
2015/1/20
Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of...
Flow Cytometry for Rapid Determination of Number of Microbial Cells Grown on Fish
flow cytometry microbial cell bacteria
2009/6/17
Application of the flow cytometry (FCM) technique for rapid determination of the total number of microbial cells grown on yellow-fin horse mackerel was described. An ultrasonic treatment was used to r...
Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid-treated mice as determined by flow cytometry
Spermatogenesis TUNEL
2009/2/18
Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by mo...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
The nuclear status of human sperm cells by TEM image cytometry：nuclear shape and chromatin texture in semen samples from fertile and infertile men
human sperm cells TEM image cytometry nuclear shape chromatin texture semen samples
2009/2/12
Changes in nuclear size, shape, and chromatin texture during spermiogenesis and epididymal transport of human sperm were recently analyzed using transmission electron microscopy (TEM) image cytome...
Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
Spermatozoa antibody infertility
2009/2/10
Detection of sperm surface antibodies is important for the diagnosis and treatment of infertility. The authors have investigated the methodologic aspects of flow cytometry (FCM) to detect sperm-bo...
Application of flow cytometry to studies on the human acrosome
flow cytometry lectin acrosome normooospermia acrosome reaction
2009/2/6
This study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a FITC-labelled plant lectin (Arachis hypogea agglutinin). Localization of the label...
DNA-flow cytometry of defined stages of rat seminiferous epithelium: effects of 3 Gy of high-energy X-irradiation
testis Sertoli germ cell
2009/2/6
Testes of adult Sprague-Dawley rats were irradiated locally by 3 Gy of 4 MeV X-rays produced by a linear accelerator. This type and dose of radiation gives an even distribution through the testis an...
Correlation between clusterin-positive spermatozoa determined by flow cytometry in bull semen and fertility
Correlation clusterin-positive spermatozoa cytometry bull semen fertility
2008/12/25
The objectives were to 1) develop a rapid and accurate method for detection of clusterin-positive spermatozoa (CPS) in bull semen and 2) determine the utility of incidence of CPS for prediction of f...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Characterizing the Glycocalyx of Poultry Spermatozoa：I. Identification and Distribution of Carbohydrate Residues Using Flow Cytometry and Epifluorescence Microscopy
Turkey chicken semen glycoconjugate lectin
2008/12/24
The aim of the present work was to use a battery of lectins to 1) delineate the carbohydrate content of sperm glycocalyx in the turkey and chicken using flow cytometry analysis, and 2) evaluate the ...
Relationship Between Sperm Viability as Determined by Flow Cytometry and Nonreturn Rate of Dairy Bulls
Spermatozoa quality insemination fertility precision accuracy
2008/12/23
A newly developed flow cytometric method for determination of sperm concentration and viability was tested in an insemination trial with cryopreserved bull sperm to establish the relationship betwee...
Assessment of Helicobacter pylori Viability by Flow Cytometry
Bacterial viability Flow Cytometry
2010/4/12
Background: Flow cytometry is a rapid, sensitive, and reliable method for determination of bacterial viability. Here we assayed the capability of flow cytometry to detect Helicobacter pylori viable ce...
Blackwell Publishing Ltd 13 C analysis of FACS-sorted cells Carbon-isotopic analysis of microbial cells sorted by flow cytometry
FACS-sorted Blackwell Publishing Ltd
2014/4/8
One of the outstanding current problems in both geobiology and environmental microbiology is the quantitative
analysis of in situ microbial metabolic activities. Techniques capable of such analysis w...