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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
ELONGASE CONDENSING ENZYME
2015/5/21
Fatty acid elongation is the extension of de novo synthesized fatty acids through a series of four reactions analogous to those of fatty acid synthase. ELOs catalyze the first reaction in the elongati...
Aerobic Uptake of Cholesterol by Ergosterol Auxotrophic Strains in Candida glabrata & Random and Site-Directed Mutagenesis of ERG25 in Saccharomyces cerevisiae
Ergosterol Candida glabrata ERG25
2015/5/22
Candida albicans and Candida glabrata are opportunistic human pathogens that are the leading cause of fungal infections, which are increasingly becoming the leading cause of sepsis in immunosuppressed...
Improvement of the Thermal Stability of a Calcium-free, Alkaline α-Amylase by Site-directed Mutagenesis
Bacillus alkaliphile α-amylase thermostability site-directed mutagenesis
2008/4/20
Alkaline α-amylase from Bacillus sp. strain KSM-K38 (AmyK38) is a calcium-free enzyme that is stable against chelating and oxidative reagents. Recently, the thermostability of this enzyme was improved...
Creation of a Novel Hydrolase by Site-directed Mutagenesis of Malto-oligosyltrehalose Synthase
trehalose malto-oligosyltrehalose synthase site-directed mutagenesis transglycosylation hydrolysis
2008/4/20
Malto-oligosyltrehalose synthase (EC 5.4.99.15, MTSase) catalyzes the conversion of α-1,4-glucan to glycosyltrehalose by forming an α,α-1,1-glucosidic linkage on the reducing side of the α-1,4-glucan....
Site-Directed Mutagenesis of Tryptophan 622 of Thermoactinomyces vulgaris R-47 Glucoamylase:pH Optima and Activities of Five Mutants
glucoamylase optimal pH catalytic base
2008/4/20
In Aspergillus awamori glucoamylase, the optimal pH has been reported to increase to maintain activity by a mutation of Ser411 which forms a hydrogen-bond with a catalytic base (Fang and Ford, Protein...
应用PCR技术对先天性长QT综合征KCNQ1基因进行定点突变的研究PCR Site-Directed Mutagenesis of Long QT Syndrome KCNQ1 Gene in vitro
长QT综合征 KCNQ1 PCR 定点突变
2008/1/20
摘要
利用聚合酶链反应（PCR）技术对长QT综合征（LQTS）KCNQ1基因进行定点突变的研究。首先设计两对引物（包含预定的突变），通过3轮PCR扩增，扩增出含有所需突变位点的片段，然后将片段克隆入T载体中，通过酶切连接的方法将突变点引入到pIRES2-EGFP-KCNQ1中，随后用Effectene转染试剂介导转染HEK293细胞。结果在真核表达载体pIRES2-EGFP-KCNQ1基础上获得...