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Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings
BY-2 cells Arabidopsis SNAP-tag live cell imaging microtubules endocytosis PIN2 auxin transporter
2023/12/21
Synthetic chemical fluorescent dyes promise to be useful for many applications in biology. Covalent, targeted labeling, such as with a SNAP-tag, uses synthetic dyes to label specific proteins in vivo ...
p24 family proteins are involved in transport to the plasma membrane of GPI-anchored proteins in plants
Arabidopsis p24 family endoplasmic reticulum glycosylphosphatidylinositol (GPI)
2023/12/19
p24 proteins are a family of type-I membrane proteins which cycle between the endoplasmic reticulum (ER) and the Golgi apparatus via Coat Protein I (COPI)- and COPII-coated vesicles. They have been pr...
Lipid Signaling through G Proteins
N-acylethanolamines G proteins lipid signaling endocannabinoids
2023/12/11
N-Acylethanolamine (NAE) signaling has received considerable attention in vertebrates as part of the endocannabinoid signaling system, where anandamide acts as a ligand for G protein–coupled cannabino...
Mapping and engineering of auxin-induced plasma membrane dissociation in BRX family proteins
BRX BREVIS RADIX (BRX) phloem development auxin-responsive biological activity
2023/12/4
Angiosperms have evolved the phloem for the long-distance transport of metabolites. The complex process of phloem development involves genes that only occur in vascular plant lineages. For example, in...
The Ca2+ sensor proteins CML37 and CML42 antagonistically regulate plant stress responses by altering phytohormone signals
Jasmonates Abscisic acid Drought Herbivory Necrotrophic pathogenes Defense Calcium Glucosinolates Spodoptera littoralis Alternaria brassicicola
2023/12/1
A transient increase in the cytosolic calcium concentration is one of the first reactions that can be measured in plant cells upon abiotic as well as biotic stress treatments. These calcium signals ar...
Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CKX proteins and cytokinin responses
proteins regulate Endoplasmic reticulum (ER) CKX proteins plant development
2023/12/1
Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Rece...
BBX19 fine-tunes the circadian rhythm by interacting with PSEUDO-RESPONSE REGULATOR proteins to facilitate their repressive effect on morning-phased clock genes
BBX19 circadian clock transcription factors zinc Arabidopsis thaliana
2023/12/1
The core plant circadian oscillator is composed of multiple interlocked transcriptional–translational feedback loops, which synchronize endogenous diel physiological rhythms to the cyclic changes of e...
南京农业大学农学院《The Plant Journal》发表郭旺珍教授团队“Cotton GhNAC4 promotes drought tolerance by regulating secondary cell wall biosynthesis and ribosomal proteins homeostasis”（图）
郭旺珍 经济作物 发育
2024/6/11
干旱是限制植物种植分布、生长发育以及作物产量的主要非生物胁迫因素之一。棉花是重要的经济作物，新疆是中国棉花种植的主要地区，占全国棉花种植面积80%以上。干旱胁迫是影响新疆棉花生产重要的非生物胁迫因素。挖掘关键抗旱基因，揭示棉花对干旱胁迫的应答机制，培育耐逆新材料和新品种，不仅具有重要的科学研究价值，也是服务棉花可持续发展的重要保障。NAC转录因子在植物非生物胁迫信号中起着重要的转录调控作用。然而，...
【分析化学学术报告】Mass Spectrometric detection of membrane-bound proteins and their noncovalent interaction partners（图）
membrane-bound proteins noncovalent
2023/6/20
【分析化学学术报告】Mass Spectrometric detection of membrane-bound proteins and their noncovalent interaction partners
A new method for analyzing protein crystals – developed by Cornell researchers and given a funky two-part name – could open up applications for new drug discovery and other areas of biotechnology and ...
学术讲座：Gastric behaviors of milk proteins for manipulating nutrient digestion（图）
学术讲座 milk proteins Nutrition Gastric behaviors
2023/7/6
学术讲座：Gastric behaviors of milk proteins for manipulating nutrient digestion。
AI system can generate novel proteins that meet structural design targets（图）
AI系统 新型蛋白质 可调蛋白质
2023/6/6
MIT researchers are using artificial intelligence to design new proteins that go beyond those found in nature.
One of the major obstacles that those conducting research on carbohydrates are constantly working to overcome is the limited array of tools available to decipher the role of sugars. As a workaround, m...
One of the hallmarks of Alzheimer’s disease is the presence of neurofibrillary tangles in the brain. These tangles, made of tau proteins, impair neurons’ ability to function normally and can cause the...
