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A microperfusion chamber for study of mammalian spermatozoa
microperfusion chamber mammalian spermatozoa
2009/2/23
The design of a microperfusion chamber is presented for use with spermatozoa or other cell suspensions. This chamber allows perfusion of a small number of spermatozoa during simultaneous observat...
Biochemical studies of metalloendoprotease activity in the spermatozoa of three mammalian species
Biochemical studies metalloendoprotease activity spermatozoa three mammalian species
2009/2/23
Ejaculated porcine and human spermatozoa, hamster spermatozoa from the cauda epididymidis, isolated hamster sperm heads and hamster cytoplasmic droplets contained activity that hydrolyzed the metall...
Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay
Acrldine orange chromatin structure SCSA TdTA
2009/2/13
Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the ...
Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei
Spermatozoa maturation fertility protamine hamster acridine orange
2009/2/11
The relationship between thiol-disulfide status and acridine orange fluorescence of testicular, epididymal, and ejaculated spermatozoa in several mammalian species was investigated. Spermatozoa were...
Cryopreservation of mammalian sperm：what we ask them to survive
cryoprotectant membrane reorganization membrane permeability membranes metabolism metabolic balance
2009/2/6
Techniques for freezing bull sperm developed over the past 40 years have not yielded protocols for preserving sperm from other species. Recent advances in our understanding of cell membrane structur...
The Multi PDZ Domain Protein MUPP1 as a Putative Scaffolding Protein for Organizing Signaling Complexes in the Acrosome of Mammalian Spermatozoa
Signal transduction acrosome reaction MPDZ
2008/12/24
Spermatozoa undergo complex sequences of precisely timed events during the process of fertilization. These priming events, which comprise capacitation, egg recognition, acrosome reaction, and sperm-...
A Flow Cytometric Method for Rapid Determination of Sperm Concentration and Viability in Mammalian and Avian Semen
Cytometry spermatozoa count vitality precision accuracy
2008/12/22
A new flow cytometric method has been developed to rapidly determine sperm concentration and viability in semen from bulls and boars. Sperm viability was determined on the basis of staining with SYB...