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Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
Long-term alterations in the permeability of the blood-testis barrier following a single intratesticular injection of dilute aqueous glycerol
Blood-testis barrier permeability glycerol seminiferous tubules Sertoli cells
2009/2/12
The mechanism whereby glycerol exerts its antispermatogenic action is not known. The objective of this study was to determine, with the use of [3H]inulin and [125I]albumin by in vivo and in vitro ...
Suppression of spermatogenesis by low-level glycerol treatment
spermatogenesis low-level glycerol treatment
2009/2/12
Previous studies have shown that a single intratesticular injection with 70% glycerol results in rapid and long-term suppression of spermatogenesis. Because of the extensive testicular damage in the...
Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant
Human sperm cryopreservation cryoprotectant sublethal cryodamage lipid peroxidation membrane stress
2009/2/12
One effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. We hypothesized two modes of suble...
Cryopreservation in different concentrations of glycerol alters boar sperm and their membranes
Swine sperm motility acrosome membrane fluidity
2008/12/29
To test the hypothesis that glycerol would concomitantly affect sperm membrane structure and the function of the intact cells, boar semen (4 ejaculates from 4 boars) was cryopreserved in an egg yo...
Glycerol disrupts tight junction-associated actin microfilaments, occludin, and microtubules in Sertoli cells
Glycerol disrupts tight junction-associated actin microfilaments occludin and microtubules in Sertoli cells
2008/12/25
Intratesticular injections of glycerol have been shown to result in a marked and prolonged reduction of spermatogenesis, accompanied by increased permeability of the blood-testis barrier. Because ...