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Synchronous assay for human sperm capacitation and the acrosome reaction
human sperm capacitation acrosome reaction
2009/2/23
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro
gossypol sperm motility sperm maturation cytoplasmic droplet capacitation acrosome reaction
2009/2/20
To determine the effects of gossypol acetate on guinea pig epididymal and vas deferens sperm maturity and in vivo susceptibility to in vitro capacitation and the acrosome reaction, we examined sperm...
The meaning of sperm capacitation. A historical perspective
sperm capacitation historical perspective
2009/2/20
It should be recalled that sperm capacitation was originally defined in 1952 as some physiological changes of the spermatozoa in the female genital tract before they are capable of penetrating and fer...
Ca2+ Is Required for Mouse Sperm Capacitation and Fertilization In Vitro
capacitation acrosome reaction whiplash motility A23187 fertilization in vitro calcium
2009/2/19
The temporal involvement of Ca2+ during mouse sperm capacitation in vitro was examined by releasing sperm into Ca2+-free medium and then introducing Ca2+ (1.80 mM, final concentration) at 30 minute ...
In Vitro Sperm Capacitation and In Vitro Fertilization with Normal Development in the Rabbit
sperm capacitation in vitro fertilization embryo culture embryo transfer spermatozoa oocytes rabbit
2009/2/19
The objective was to obtain in vitro sperm capacitation and fertilization to approximate the normal in vivo processes. Rabbit oocytes from follicles, ovarian surfaces, and oviducts were fertilized...
Interaction between Ca2+, cyclic 3',5' adenosine monophosphate, the superoxide anion, and tyrosine phosphorylation pathways in the regulation of human sperm capacitation
Calmodulin phosphatase kinase
2009/2/17
In order to fertilize the egg, spermatozoa must go through the capacitation process where they experience Ca2+ uptake, increases in cyclic 3',5' adenosine monophosphate (cAMP) concentrations, supe...
Human sperm capacitation induced by biological fluids and progesterone, but not by NADH or NADPH, is associated with the production of superoxide anion
Spermatozoa MCLA reactive oxygen species sperm hyperactivation protein tyrosine
2009/2/17
Recent evidence indicated that human sperm capacitation is associated with an increased production of superoxide anion (O2.-). To further study the role and importance of O2.- in capacitation, we in...
Premature capacitation of bovine spermatozoa is initiated by cryopreservation
Premature capacitation bovine spermatozoa cryopreservation
2009/2/17
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Compromised sperm protein phosphorylation after capacitation, swim-up, and zona pellucida exposure in teratospermic domestic cats
Cat sperm teratospermia tyrosine phosphorylation zona pellucida
2009/2/16
Tyrosine phosphorylated proteins recently have been found in mouse and human spermatozoa. Our objectives were to (1) determine if domestic cat spermatozoa also express tyrosine phosphorylated pro...
Low levels of nitric oxide promote human sperm capacitation in vitro
NONOates reactive oxygen species catalase hydrogen peroxide peroxinitrite nitric oxide synthase
2009/2/13
The influence of nitric oxide on human sperm hyperactivation and capacitation, as well as its mechanism of action and its possible origin from spermatozoa were studied. Percoll-washed spermatozoa ...
Release of angiotensin-converting enzyme（ACE）from human spermatozoa during capacitation and acrosome reaction
ACE content ejaculate kininase II
2009/2/13
The present study examines the release of angiotensin-converting enzyme (ACE) from human spermatozoa during capacitation conditions and in correlation to acrosome reaction and cell death. The ACE ...
Capacitation in vitro of stallion spermatozoa：comparison of progesterone-induced acrosome reactions in fertile and subfertile males
Equine male gamete infertility acrosomal
2009/2/13
Mammalian sperm that have completed capacitation are capable of undergoing the acrosome reaction in response to a number of biological and chemical stimuli. In the present report, we have investig...
Development of computer-directed methods for the identification of hyperactivated motion using motion patterns developed by rabbit sperm during incubation under capacitation conditions
computer-directed methods identification hyperactivated motion motion patterns rabbit sperm
2009/2/12
Rabbit spermatozoa developed motions that mimicked hyperactivated motility during incubation for 16-20 hours under capacitation conditions and in several other commonly used media. Sperm from some rab...
Effect of follicular or oviductal fluids on movement characteristics of bovine sperm during capacitation in vitro
Sperm motion analysis motility hyperactivation
2009/2/12
Mammalian sperm exhibit characteristic motility changes associated with capacitation. Movement characteristics of bovine sperm incubated in noncapacitating (control, medium alone), capacitating (ovidu...