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Electron microscopic study of the secretion process in bovine reproductive organs
Electron microscopic study secretion process bovine reproductive organs
2009/2/23
The origin and mechanism of the secretion of membrane-bound particles in bovine seminal plasma were studied with transmission (TEM) and scanning (SEM) electron microscopy of the epididymis, vas defe...
Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility
Quantification bovine sperm separation swim-up method
2009/2/23
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
Low molecular weight FSH binding inhibitor in bovine testis
Low molecular weight FSH bovine testis
2009/2/23
Purification of low Mr (less than 5000) inhibitors of FSH binding to receptors has been hampered by their low concentration in tissue extracts and physiologic fluids. The calf testis represents an a...
Comparison of the penetration ability of human spermatozoa into bovine cervical mucus and zona-free hamster eggs
penetration ability human spermatozoa bovine cervical mucus zona-free hamster eggs
2009/2/20
In vitro bovine cervical mucus (BCM) penetration tests, sperm penetration assays (SPA) using zona-free hamster eggs, and routine semen analyses were performed on a total of 136 freshly collected sem...
Bovine sperm adenylate cyclase. Inhibition by adenosine and adenosine analogs
adenosine epididymal spermatozoa adenylate cyclase inhibition P-site
2009/2/20
Since both adenosine and cAMP affect sperm motility and metabolism, the authors have studied the mode of interaction of adenosine with the adenylate cyclase activity in either the presence or the ab...
Human sperm penetration in bovine cervical mucus. Clinical studies I
Human sperm penetration bovine cervical mucus
2009/2/19
Penetration of spermatozoa in bovine cervical mucus (BCM) from two groups of men was investigated and correlated with the different parameters of spermograms from these same subjects. Sperm penetr...
A Method for Improvement of Sperm Quality of an Ejaculated Human Specimen Using Bovine Cervical Mucus as a Biological Filter
teratozoospermia bovine cervical mucus penetration test
2009/2/19
A method is described for improving the quality of semen from subfertile men. Semen samples were filtered through bovine cervical mucus (collected at estrus) into spermatozoa-free seminal plasma (fr...
In Vitro Gonadotropin Stimulation of Bovine Sertoli Cell Ornithine Decarboxylase Activity
ornithine decarboxylase Sertoli cell gonadotropins bovine calf
2009/2/19
The activity of ornithine decarboxylase (ODC) was determined in primary cultures of bovine Sertoli cells after gonadotropin stimulation in vitro. A significant increase in both ODC activity and putr...
Nature of the Clearance of Exogenous Testosterone and Progesterone in Ejaculated Bovine Semen
progesterone testosterone blood semen ejaculated
2009/2/19
Plasma and semen concentrations of androgens and progestins were not influenced by repetitive ejaculation in bulls. Within 20 minutes after the injection of testosterone (100 mg Intravenously), plas...
Relationship of Spermatozoal Migration in Cervical Mucus to Bovine Fertility
spermatozoa bovine cervical mucus sperm migration fertility
2009/2/19
Frozen bovine semen from males of well-established, generally high fertility levels was thawed and used in three experiments for in vitro capillary tube migration through cervical mucus. Bovine cerv...
Comparison of fresh and cryopreserved human sperm attachment to bovine oviduct（uterine tube）epithelial cells in vitro
Sperm coculture sperm storage artificial insemination
2009/2/18
Formation of a prefertilization sperm reservoir in mammals is thought to occur via sperm cell attachment to fallopian tube or oviduct epithelial cells (OEC). Recent data suggests that such an interact...
Single-step isolation of bovine seminal platelet-activating factor acetylhydrolase
Seminal plasma phospholipase A2 protein purification
2009/2/17
Bovine seminal platelet-activating factor acetylhydrolase was isolated to >90% purity in a single step using a butyl sepharose column. The procedure involves the elution of the activity by use of ...
Catalase and oviductal fluid reverse the decreased motility of bovine sperm in culture medium containing specific amino acids
Hydrogen peroxide oviduct secretions toxicity
2009/2/17
The motility and velocity of bovine spermatozoa incubated in TCM-199 are reduced in comparison with those incubated in a simpler media made for sperm, such as modified Tyrode (Sp-Talp). Moreover, ...
Partial purification and localization of platelet-activating factor acetylhydrolase from bovine seminal plasma
Partial purification platelet-activating factor bovine seminal plasma
2009/2/17
Platelet-activating factor (PAF) is a potent lipid mediator that is inactivated by platelet-activating factor acetylhydrolase (PAF-AH). Platelet-activating factor bioactivity has been detected in ...
Premature capacitation of bovine spermatozoa is initiated by cryopreservation
Premature capacitation bovine spermatozoa cryopreservation
2009/2/17
Poor motility and abnormal acrosomal morphology only partially explain the reduced fertility of cryopreserved bovine spermatozoa. To test the hypothesis that cryopreservation procedures (dilution,...