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Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Electrophoretic and Chromatographic Properties of Boar Sperm Plasma Membranes：Antigens and Polypeptides with Affinity for Isolated Zonae Pellucidae
sperm plasma membrane zonae pellucidae
2009/2/19
Two methods were used to assess the ability of plasma membrane (PM) polypeptides to bind to isolated zonae pellucidae (ZP). Crossed immunoelectrophoresis patterns of detergent solubilized PM were co...
Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation
Extender components surfactants boar sperm function membrane behavior cryopreservation
2009/2/18
To determine how the individual components of extenders affected boar sperm function and membrane structure and to test a new surfactant's cryoprotective ability, boar sperm were cryopreserved in ...
Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations：results of two fertility trials
Semen analysis CASA spermatozoa
2009/2/17
Two fertility trials were undertaken to evaluate the relationship between boar semen quality and fertility (conception rate and litter size) after on-farm artificial insemination (AI). Trial 1 inclu...
Comparative study of boar sperm coming from the caput, corpus, and cauda regions of the epididymis
boar sperm caput corpus cauda regions epididymis
2009/2/13
Sperm quality in the caput, corpus, and cauda regions of the epididymis of healthy and sexually mature Landrace boars was studied. Epididymal sperm characteristics were examined by light microscopy (L...
Movement characteristics of boar sperm obtained from the oviduct or hyperactivated in vitro
Pig uterine tube fallopian tube sperm motility
2009/2/10
The objectives of this study were to describe hyperactivated motility in boar sperm and to determine the incidence of hyperactivation among boar sperm flushed from the oviduct. Oviducts were surgica...
Cryopreservation in different concentrations of glycerol alters boar sperm and their membranes
Swine sperm motility acrosome membrane fluidity
2008/12/29
To test the hypothesis that glycerol would concomitantly affect sperm membrane structure and the function of the intact cells, boar semen (4 ejaculates from 4 boars) was cryopreserved in an egg yo...
Effects of Seminal Plasma on Cooling-Induced Capacitative Changes in Boar Sperm
Cryopreservation semen capacitation
2008/12/24
Porcine seminal plasma (SP) has been shown to contain factors that have a decapacitative or capacitation-inhibiting effect on sperm. The objectives of the present study were to compare the capaci...
Dissecting the Protective Effect of the Seminal Plasma Spermadhesin PSP-I/PSP-II on Boar Sperm Functionality
Boar seminal plasma spermadhesin PSP-I/PSP-II protective effect on sperm function sperm survival highly diluted spermatozoa
2008/12/24
To dissect the protective activity of PSP-I/PSP-II, the effect of the isolated subunits PSP-I and PSP-II and their affinity-purified tryptic peptide and glycan fractions on the viability, mitochondr...
Effects of Centrifugation Before Freezing on Boar Sperm Cryosurvival
Cryopreservation g-force frozen-thawed sperm semen quality
2008/12/22
Current protocols for boar sperm cryopreservation require the centrifugation of semen in order to separate sperm cells from the seminal plasma. This study evaluated the influence of different cent...
The Importance of Calcium in the Appearance of p32，a Boar Sperm Tyrosine Phosphoprotein，During In Vitro Capacitation
Tyrosine phosphorylation chelator A23187 indo-1
2008/12/19
After ejaculation, mammalian sperm must undergo a preparation period known as "capacitation" to become capable of fertilizing the oocyte. Although physiological capacitation occurs in the female g...
Involvement of Cytoplasmic Free Calcium in Boar Sperm: Head-to-Head Agglutination Induced by a Cell-Permeable Cyclic Adenosine Monophosphate Analog
Capacitation BAPTA thapsigargin fluo-3 Ca2+-ATPase
2008/12/18
When boar spermatozoa are incubated in a medium designed for in vitro fertilization, many of them become agglutinated at the acrosomes. We previously reported that bicarbonate and cyclic adenosine...