搜索结果: 1-12 共查到“医学 CMV”相关记录12条 . 查询时间(0.145 秒)
CMV Amplifies T-cell Redeployment to Acute Exercise Independently of HSV-1 Serostatus
CYTOMEGALOVIRUS HERPESVIRUS IMMUNESYSTEM PHYSICALACTIVITY LYMPHOCYTE
2017/5/18
Latent cytomegalovirus (CMV) infection has been shown to alter the lymphocyte response to acute aerobic exercise, likely due to the corresponding increase in exercise-responsive memory CD8+ T cells. I...
hTERT|CEA及CMV启动子在人结肠癌细胞株中的转录活性比较
结肠肿瘤 转录启动子 转染
2014/4/28
比较人端粒酶催化亚单位（hTERT），癌胚抗原（CEA）及巨细胞病毒（CMV）启动子在人结肠癌细胞株LoVo和SW480中的转录活性。 方法：设计引物应用PCR法从人结肠癌基因组中克隆hTERT和CEA启动子；用双酶切和PCR法切除原始载体pLVX-EGFP-3FLAG中的CMV启动子后，将hTERT，CEA启动子与该载体重组，构建出重组质粒pLVX-hTERTp-EGFP-3FLAG和pLVX-...
CMV启动子序列检测的复合实时定量PCR方法的建立
CMV启动子 序列检测 PCR方法
2013/11/29
建立和验证用于巨细胞病毒(CMV)启动子序列检测的复合定量PCR方法。方法 以CMV 启动子序列和小鼠管家基因β-actin的cDNA序列为模版分别设计探针和引物,用SYBR GreenⅠ熔解曲线分析两对引物扩增的特异性。对反应体系进行系统优化,并验证检测方法的灵敏度、线性和重复性、以及复合反应效率的变化。结果 针对CMV启动子序列的上游引物为5'AGACTTGGAAATCCCCGTGAGT3',...
探讨CMV包膜糖蛋白B（CMVgB）基因分型对造血干细胞移植后CMV感染患者病毒载量和抗病毒疗程的影响。方法 选择接受造血干细胞移植且CMV-DNA阳性的115例CMV感染患者，采用荧光定量PCR检测病毒载量，限制性片段长度多态性PCR(PCR-RFLP)法鉴定CMVgB基因分型，分析不同CMVgB基因型感染患者病毒载量和抗病毒疗程的差异。结果 ①115例患者CMVgB基因型分布：gB1型42...
The Use of pp150 and gp116 Synthetic Peptides in the Detection of CMV Antibodies
CMV IgM IgG Fusion protein Peptides pp150 gp116 gp H
2015/9/22
Background: Human cytomegalovirus (HCMV) infection may cause severe outcomes in transplant recipients and pregnant women. Diagnosis of CMV infections by using serological detection of CMV specific ant...
CMV Colitis Presenting as Neoplastic Lesions with Life-Threatening Bleeding in an Immunocompetent Patient
CMV colitis Immunocompetent Patient
2015/9/21
An elderly woman with multiple medical problems developed cytomegalovirus (CMV) colitis following a prolonged hospitalization. She presented life–threatening colonic bleeding mimicking a neoplastic pr...
Serological ELISA Test (IgM & IgG) for Prospective Study of Cytomegalovirus (CMV) Infection in Pregnant Women
Cytomegalovirus Congenital Disorders Maternal infection Iran
2009/11/25
Background: Cytomegalovirus (CMV) infection is associated with significant maternal and fetal consequences. The aim of present study was to determine the current prenatal CMV seroprevalence in Ea...
Viral coinfection in the oral cavity of HIV-infected children: relation among HIV viral load,CD4+T lymphocyte count and detection of EBV,CMV and HSV
AIDS-related opportunistic infections Herpes simplex virus Cytomegalovirus Epstein-Barr virus Viral load CD4-positive T-lymphocytes
2009/10/20
Viral coinfection in the oral cavity associated to HIV infection was evaluated in 180 children from birth to 13 years of age of both sexes. The oral examinations were performed at the Pediatric AIDS O...
HRE/CMV启动子的克隆和缺氧调控活性的测定
缺氧反应元件 荧光素酶 基因 报告
2009/6/9
目的： 将具有缺氧调控作用的多种缺氧反应元件 (HRE)与CMV启动子组合，以获得缺氧应答启动子。将荧光素酶报告基因置于缺氧应答启动子的下游，通过检测荧光素酶活性的方法，方便地研究缺氧应答启动子缺氧诱导的作用。方法: 合成多种HRE核苷酸序列，并设立HRE突变对照，克隆入pCI-neo中，构建HRE/CMV启动子。扩增HRE/CMV序列，定向亚克隆入pGL3-Basic中，获得HRE/CMV荧光素...
HPV、HSV和CMV感染与宫颈癌的关系
宫颈癌 人乳头瘤病毒 原位杂交
2008/10/27
背景与目的： 探讨宫颈癌前病变和宫颈癌的发生发展与人乳头状瘤病毒及单纯疱疹病毒(Herpes Simplex Virus,HSV)、巨细胞病毒(Cytomegalovirus,CMV)的关系。 材料与方法： 对81例不同宫颈病变组织进行HPV16/18和HPV6/11原位杂交，同时对103例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。 结果: 病毒DNA原位杂交信号的分布与HE染色中...
人DDR2基因pShuttle-CMV穿梭载体的构建及表达。