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Exposure of thawed frozen bull sperm to a synthetic peptide before artificial insemination increases fertility
Enhancement of fertility bovine sperm prosaposin
2009/2/18
We evaluated the effect on fertility of in vitro exposure of thawed frozen bull sperm to synthetic FertPlus peptide prior to artificial insemination (AI). The peptide represented a 60-amino acid s...
Exposure of human, boar, or bull sperm to a synthetic peptide increases binding to an egg-membrane substrate
human boar bull sperm synthetic peptide increases egg-membrane substrate
2009/2/18
We evaluated the effects of in vitro exposure of sperm to synthetic FertPlus peptide, which represents a 60-amino acid sequence within rat prosaposin, using a microwell sperm-binding assay (SBA), ...
Artificial insemination using cryopreserved semen is a common management tool of the contemporary livestock producer. However, cryopreservation is detrimental to sperm function and fertility, kill...
Use of Hoechst 33342 stain to evaluate live fresh and frozen bull sperm by computer-assisted analysis
Sperm motility DNA ultraviolet illumination
2009/2/17
The objective of this research was to investigate possible procedures for evaluating living bull sperm stained with Hoechst 33342 while in a simple medium and in commonly used complex egg yolk-glyce...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies：I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...
Media and dilution procedures tested to minimize handling effects on human, rabbit, and bull sperm for computer-assisted sperm analysis（CASA）
CASA holding time hyperactivation TALP PBS
2009/2/16
Proper handling of semen prior to computer-assisted sperm analysis (CASA) is critical if the analysis is to be representative of the fresh sample. The effects of diluting medium or dilution and ho...
Cryopreservation alters the levels of the bull sperm surface protein P25b
Cryopreservation bull sperm surface protein P25b
2008/12/25
Fertility of frozen-thawed bull sperm is reduced by cryopreservation. Freezing-thawing procedures can result in as much as a sevenfold fertility decrease. Sperm mortality and loss of motility do not...