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Changes of Hypothalamic GnRH-I, POMC and NPY mRNA Expression and Serum IGF-I and Leptin Concentrations during Maturation of Shaoxing Ducks (Anas platyrhynchos)
Changes of Hypothalamic GnRH-I, POMC and NPY mRNA Expression and Serum IGF-I and Leptin Concentrations during Maturation of Shaoxing Ducks (Anas platyrhynchos)
2016/5/6
Sexual maturity in poultry is controlled by a complex neural circuit located in the basal forebrain, which integrates the central and peripheral signals to activate hypothalamic gonadotrophin-releasin...
Nutritional Modulation of Leptin Messenger RNA in Broiler Chickens
Animal Nutrition Life Science Graduate School of Agriculture Tohoku University
2009/7/31
Three sets of experiments were conducted to investigate the nutritional modulation of leptin mRNA expression in chicken adipose tissue and liver. Chicken leptin expression in adipose tissue and liver ...
Leptin Signaling and Action in Birds
avian leptin receptor signal transduction physiological action
2009/7/30
The discovery of leptin has led to an understanding of the regulation of energy expenditure and food intake in a number of vertebrates. Leptin is also implicated in diverse physiological functions. In...
①取20只240日龄的粤黄鸡种母鸡平分为处理组和对照组，分别在第3、31、63和84 d对之接种鸡Leptin重组融合蛋白(处理组)或BSA(对照组)1 mL/羽。每天记录产蛋率，每10天测定采食量，每两周称重，在试验的第1、31、53、78和91 d采血。结果表明：Leptin处理组的血浆抗Leptin抗体水平和Leptin水平在免疫后显著高于对照组(P<0.01)；第3和4次免疫后处理组产蛋率...
鸭leptin基因在大肠杆菌中的融合表达及生物学活性分析
鸭 leptin基因 基因表达 生物学活性
2008/9/1
【目的】研究鸭leptin在体外高效表达特点，探讨表达产物的生物学活性及其功能。【方法】构建重组鸭leptin基因原核表达菌株，重组菌株经不同浓度IPTG和不同时间诱导后，对表达蛋白进行提取、纯化、复性和浓缩，经注射昆明小鼠后，对小鼠的体重﹑采食量和体脂含量进行分析。【结果】鸭leptin在大肠杆菌中实现了高效特异性融合表达，融合蛋白分子量约为20 kD，其中16 kD为鸭leptin基因表达产物...
Leptin基因对陆川猪和大白猪产仔数的影响
产仔数 大白猪 陆川猪 Leptin基因
2008/4/8
根据leptin基因在GenBank中的已知序列设计两对引物,采用PCR-SSCP技术在陆川猪和大白猪群体中进行单核苷酸多态性(SNPs)检测,发现了两个SNPs位点,并对其不同基因型个体PCR回收产物进行测序，同时对这两个SNPs位点与产仔数进行了关联分析。结果表明：这两个SNPs位点分别是在第3 469碱基处发生了T→C突变和第3 714碱基处发生了T→G突变，这两个突变都是同义突变；T371...
不同能量摄入水平对奶牛产乳性能及血液中Leptin和NPY浓度的影响研究
能量奶牛 产乳性能
2008/3/3
将30头围产期健康奶牛随机分为3组，每组均为10头，其中Ⅰ组为对照组，Ⅱ组和Ⅲ组为试验组；Ⅰ、Ⅱ、Ⅲ组分别于产前第28 d开始饲喂标准日粮（能量摄入100%组）、标准日粮增加20%能量（能量摄入120%组）和标准日粮减少20%能量（能量摄入80%组），产后各组奶牛均饲喂标准泌乳日粮，至产后第56 d结束，观察不同能量摄入水平对产后奶牛产乳性能及血液中瘦素（Leptin）和神经肽Y(NPY)浓度的...
Leptin Is a Metabolic Signal for GnRH-LH/FSH Axis in Feed-restricted Ewes
Leptin Gonadotropin Ewe Metabolic Signal
2016/4/26
Two experiments were conducted to determine whether leptin is a metabolic signal for gonadotropin secretion in ewes. In the first experiment, twenty-eight cyclic Chal ewes were assigned randomly to an...
Cloning and Expression of the Duck Leptin Gene and the Effect of Leptin on Food Intake and Fatty Deposition in Mice
Duck Leptin Expression Food Intake Fatty Deposition
2016/4/25
Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of le...
Characterization of Leptin Levels in Gestating Callipyge Ewes
Adipose Callipyge Leptin Sheep
2016/4/25
The callipyge mutation in sheep is a polar overdominant mutation that results in post-natal muscle hypertrophy in the loin and hindquarters of paternal heterozygotes (+/CLPG). Sheep that are homozygou...
Leptin基因对陆川猪和大白猪产仔数的影响
leptin 陆川猪 大白猪 产仔数
2013/12/27
根据leptin基因在GenBank中的已知序列设计两对引物,采用PCR-SSCP技术在陆川猪和大白猪群体中进行单核苷酸多态性(SNPs)检测,发现了两个SNPs位点,并对其不同基因型个体PCR回收产物进行测序，同时对这两个SNPs位点与产仔数进行了关联分析。结果表明：这两个SNPs位点分别是在第3 469碱基处发生了T→C突变和第3 714碱基处发生了T→G突变，这两个突变都是同义突变；T371...
Plasma Leptin and Performance of Purebred and Backcrossed Hereford throughout Grazing and Feedlot Fattening
Feedlot Grazing Hereford Japanese Black Leptin
2016/4/15
In a herd of 24 spring-born steers, plasma leptin and performance of selected purebred (n=5) and backcrossed Hereford (n=5) were compared in a year-round summer grazing and winter feedlot fattening. B...