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Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination
Self-incompatibility Prunus avium S-RNase AS-PCR pollination test
2011/1/10
Interspecific Crossing between Sweet Cherry (Prunus avium L.) and Ornamental Cherry (Prunus × yedoensis Matsum.)
cross compatibility embryo culture interspecific hybrid morphological traits RAPD-analysis
2009/7/9
This report illustrates interspecific crossings between 7 cultivars of sweet cherries (P. avium L.) and 1 cultivar of ornamental cherry (P. × yedoensis Matsum.). Our objective is to obtain hybrids tha...
甜樱桃 (Prunus avium)品种 S基因型鉴定
甜樱桃 自交不亲和 S-RNase基因(S 基因 ) S基因型
2008/1/25
摘要 根据蔷薇科S-RNase基因(S基因)高度保守区C2 和RC4区设计一对特异引物PruC2 和PruC4R,对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。克隆S基因的扩增片段,核酸序列在GenBank 上搜索,确定了4种S基因扩增片段的核酸序列和大小。结果表明,在琼脂糖凝胶上位置相同的扩增带其核酸序列相同,是同一种S基因。4种S基因扩增片段的大小分别是: S1 为 677 bp, S...