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鸡Visfatin蛋白的原核表达、纯化及其活性研究
鸡重组Visfatin 克隆 原核表达 条件优化 3T3-L1
2016/10/31
本试验旨在通过克隆Visfatin基因，将其在原核表达系统中表达并纯化，通过3T3-L1细胞的分化验证其活性，成功获得鸡重组Visfatin蛋白。以AA肉仔鸡肝组织总RNA为模板，qRT-PCR扩增Visfatin基因，转化进入pGEM-T载体，再与原核表达载体pET-30a连接转化，获取含pET-30a-Visfatin重组质粒的E.coli BL21(DE3)表达菌株。以IPTG诱导重组菌株...
Limiting Concentrate during Growing Period Affect Performance and Gene Expression of Hepatic Gluconeogenic Enzymes and Visfatin in Korean Native Beef Calves
Calf, Concentrate, Growth, Gluconeogenic Enzymes, Plasma
2016/5/11
This study elucidated the effects of limited concentrate feeding on growth, plasma profile, and gene expression of gluconeogenic enzymes and visfatin in the liver of Hanwoo beef calves. The purpose of...