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New microscope technique reveals internal structure of live embryos（图）
New microscope technique internal structure live embryos
2017/9/4
University of Illinois researchers have developed a way to produce 3-D images of live embryos in cattle that could help determine embryo viability before in vitro fertilization in humans.Infertility c...
Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Myostatin Zinc-finger Nucleases Knockout, Sheep
2016/11/10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause “double-muscling” trait in animals. In order to block the inhibiting effect of M...
Green Light-emitting Diodes Light Stimuli during Incubation Enhances Posthatch Growth without Disrupting Normal Eye Development of Broiler Embryos and Hatchlings
Chick Embryo Incubation Light Emitting Diodes Light Stimuli
2016/11/10
Monochromatic green light-emitting diodes (LED) light stimuli influences the posthatch growth performance of chicks. This study was undertaken with the following objectives: i) to examine whether the ...
Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
activin A SCNT embryo development bovine
2015/6/8
To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early developmen...
Hypodiploidy as a prominent attributor to chromosomal aneuploidy in transgenic rabbit embryos
chromosomal aneuploidy EGFP embryo hFVIII rabbit transgenic
2015/6/5
Transgenic animals play a vital role in basic research, agriculture and pharmaceutical industries. Rabbits have the advantage of other large laboratory species in that they have a short gestation peri...
Comparison of two vitrification methods for cryopreservation of porcine embryos
pig embryo transfer
2015/6/1
The aim of this study was to compare two vitrification methods of porcine perihatching blastocysts with regard to the success of transfer of these embryos to the recipients. Expanded, hatching, or hat...
Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos
laparoscopic insemination embryo diversity pig
2015/4/16
Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. Howe...
Conception rate after sex determination and cryopreservation of D7 bovine embryos
cattle superovulation sexing
2015/4/8
The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D7) bovine embryos and to compare it with transfer of fresh female embryos. High quality...
Lower quality bovine embryos may be successfully used for sex determination
cattle embryo superovulation sex determination polymerase chain reaction embryo transfer
2015/3/13
The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well a...
The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos
bovine embryo superovulation cryopreservation freezing thawing vitrification embryo transfer
2015/3/5
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a...
Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation
follicular development bovine oocyte in vitro embryos cryopreservation
2015/3/5
The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and...
Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
Knockout Pig Reporter Based Enrichment Somatic Cell Nuclear Transfer Zinc Finger Nuclease
2016/5/16
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to cr...
Comparative Analysis of Nkx2-5/GATA4/TBX5 Expression in Chicken, Quail and Chicken-quail Hybrids during the Early Stage of Cardiac Development in Embryos
Cardiac Development Embryogenesis Gene Expression Chicken Quail Chicken-quail Hybrids
2016/5/11
The present study makes an investigation into expression of genes related to cardiac development in chicken, quail and chicken-quail hybrids during the early stage of embryogenesis. Real-time PCR was ...
Altered pathogenicity, immunogenicity, tissue tropism and 3'-7 kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
vian infectious bronchitis coronavirus Pathogenicity Immunogenicity Tissue tropism Genome sequence Serial passage
2016/12/20
In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of ...
Transient GUS and GFP Expression in Spanish Red Cedar (Cedrela odorata L.) Somatic Embryos. Optimization of Bombardment Conditions and Evaluation of Selective Agent Lethal Dose
Biolistics Genetic Transformation Tree Genetic Modification Tropical Wood
2013/3/4
Cedrela odorata is a tropical tree widely appreciated for its wood. Commercial plantations are frequently hampered by the attack of the meliacea borer, Hypsipyla grandella, and the lack of resistant v...