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Effect of ZnO nanoparticle on cell viability,zinc uptake efficiency,and zinc transporters gene expression:a comparison with ZnO and ZnSO4
nano-ZnO bulk ZnO zinc transportation TPEN IEC-6 cells
2017/2/21
Zinc plays an important role in functional and structural integrity of cells. The aim of the current study was to compare cell viability, zinc uptake efficiency, and gene expression of metallothionein...
In a possible step forward for gene therapy,Stanford researchers made mice glow like fireflies
step forward gene therapy Stanford researchers mice glow fireflies
2017/3/14
Timothy Blake, a postdoctoral fellow in the Waymouth lab, was hard at work on a fantastical interdisciplinary experiment. He and his fellow researchers were refining compounds that would car...
Generation of Fibroblasts Lacking the Sal-like 1 Gene by Using Transcription Activator-like Effector Nuclease-mediated Homologous Recombination
Transcription Activator-like Effector Nuclease [TALEN] Sal-like 1 Gene
2016/5/20
The Sal-like 1 gene (Sall1) is essential for kidney development, and mutations in this gene result in abnormalities in the kidneys. Mice lacking Sall1 show agenesis or severe dysgenesis of the kidneys...
Knock-in of Enhanced Green Fluorescent Protein or/and Human Fibroblast Growth Factor 2 Gene into β-Casein Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein
Knock-in Homologous Recombination Therapeutic Proteins Porcine β-casein Gene
2016/5/17
Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjectio...
Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (NRAMP1) and Its Association with Immune Traits in Pigs
Natural Resistance-associated Macrophage Protein 1 (NRAMP1) Polymorphism Expression Association
2016/5/17
Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune c...
Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation
Peripheral Blood Mononuclear Cells (PBMC) Polyinosinic:Polycytidylic Acid
2016/5/16
Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the...
Swine Leukocyte Antigen-DQA Gene Variation and Its Association with Piglet Diarrhea in Large White, Landrace and Duroc
SLA DQA Gene Genetic Polymorphism Piglet Diarrhea
2016/5/12
The swine leukocyte antigen class II molecules are possibly associated with the induction of protective immunity. The study described here was to investigate the relationship between polymorphisms in ...
Regulation of Interferon-stimulated Gene (ISG)12, ISG15, and MX1 and MX2 by Conceptus Interferons (IFNTs) in Bovine Uterine Epithelial Cells
IFNT Isoforms ISG MX Uterus
2016/5/11
Various endometrial genes in ruminant ungulates are regulated by conceptus interferon tau (IFNT). However, the effect of each IFNT isoform has not been carefully evaluated. In this study, the effects ...
Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene
Cattle Meat Sex Determination Meat Quality DEAD Box Protein Gene
2016/5/10
Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also fem...
Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast Cells
fat1 Gene Expression Catalysis Efficiency Bovine Fetal Fibroblast Cells
2016/5/9
The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs ...
Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)
Cashmere Goat IGFBP-5 Expression Pattern Tissue-specific Hair Gene
2016/5/9
Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore ...
Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2016/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Melatonin Attenuates Nitric Oxide Induced Oxidative Stress on Viability and Gene Expression in Bovine Oviduct Epithelial Cells, and Subsequently Increases Development of Bovine IVM/IVF Embryos
Melatonin Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Nitric Oxide Antioxidant
2016/5/6
The objective of the present study was to elucidate the fundamental mechanism of bovine oviduct epithelial cell (BOEC) co-culture on developmental capacity of bovine IVM/IVF embryos and to determine w...
PCR-SSCP of Serum Lysozyme Gene (Exon-III) in Riverine Buffalo and Its Association with Lysozyme Activity and Somatic Cell Count
Polymorphism Lysozyme Gene SSCP Buffalo Association SCC Sequencing
2016/5/5
Serum lysozyme gene is one of the important genes influencing the immune system as its product can cause lysis of bacterial cell wall by cleaving the peptidoglycan layer. The present investigation on ...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine
Suppression Subtractive Hybridization (SSH) Gene Expression Cumulus Cells Porcine
2016/5/3
In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communication...